Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation

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Ding , X , Willenborg , S , Bloch , W , Wickström , S A , Wagle , P , Brodesser , S , Roers , A , Jais , A , Bruening , J C , Hall , M N , Rueegg , M A & Eming , S A 2020 , ' Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation ' , Journal of Allergy and Clinical Immunology , vol. 145 , no. 1 , pp. 283-+ . https://doi.org/10.1016/j.jaci.2019.07.033

Title: Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation
Author: Ding, Xiaolei; Willenborg, Sebastian; Bloch, Wilhelm; Wickström, Sara A.; Wagle, Prerana; Brodesser, Susanne; Roers, Axel; Jais, Alexander; Bruening, Jens C.; Hall, Michael N.; Rueegg, Markus A.; Eming, Sabine A.
Contributor: University of Helsinki, Helsinki Institute of Life Science HiLIFE
Date: 2020-01
Language: eng
Number of pages: 26
Belongs to series: Journal of Allergy and Clinical Immunology
ISSN: 0091-6749
URI: http://hdl.handle.net/10138/323976
Abstract: Background: Perturbation of epidermal barrier formation will profoundly compromise overall skin function, leading to a dry and scaly, ichthyosis-like skin phenotype that is the hallmark of a broad range of skin diseases, including ichthyosis, atopic dermatitis, and a multitude of clinical eczema variants. An overarching molecular mechanism that orchestrates the multitude of factors controlling epidermal barrier formation and homeostasis remains to be elucidated. Objective: Here we highlight a specific role of mammalian target of rapamycin complex 2 (mTORC2) signaling in epidermal barrier formation. Methods: Epidermal mTORC2 signaling was specifically disrupted by deleting rapamycin-insensitive companion of target of rapamycin (Rictor), encoding an essential subunit of mTORC2 in mouse epidermis (epidermis-specific homozygous Rictor deletion [Ric(EKO)] mice). Epidermal structure and barrier function were investigated through a combination of gene expression, biochemical, morphological and functional analysis in Ric(EKO) and control mice. Results: Ric(EKO) newborns displayed an ichthyosis-like phenotype characterized by dysregulated epidermal de novo lipid synthesis, altered lipid lamellae structure, and aberrant filaggrin (FLG) processing. Despite a compensatory transcriptional epidermal repair response, the protective epidermal function was impaired in Ric(EKO) mice, as revealed by increased transepidermal water loss, enhanced corneocyte fragility, decreased dendritic epidermal T cells, and an exaggerated percutaneous immune response. Restoration of Akt-Ser473 phosphorylation in mTORC2-deficient keratinocytes through expression of constitutive Akt rescued FLG processing. Conclusion: Our findings reveal a critical metabolic signaling relay of barrier formation in which epidermal mTORC2 activity controls FLG processing and de novo epidermal lipid synthesis during cornification. Our findings provide novel mechanistic insights into epidermal barrier formation and could open up new therapeutic opportunities to restore defective epidermal barrier conditions.
Subject: Epidermal barrier
mammalian target of rapamycin complex 2
Rictor
ichthyosis
filaggrin
epidermal lipid synthesis
STRATUM-CORNEUM BARRIER
OF-FUNCTION MUTATIONS
CAUSE ICHTHYOSIS
STEM-CELLS
T-CELLS
IN-VIVO
DIFFERENTIATION
AKT
EXPRESSION
ABNORMALITIES
3121 General medicine, internal medicine and other clinical medicine
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