Determination of the intramammary dose of benzylpenicillin required to maintain an adequate concentration in the milk to inhibit Gram-positive bacteria in the clinically normal udder for 24 hr

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Abbeloos , E , Pyörälä , S , Rajala-Schultz , P & Myllys , V 2018 , ' Determination of the intramammary dose of benzylpenicillin required to maintain an adequate concentration in the milk to inhibit Gram-positive bacteria in the clinically normal udder for 24 hr ' , Journal of Veterinary Pharmacology and Therapeutics , vol. 41 , no. 5 , pp. 691-698 . https://doi.org/10.1111/jvp.12671

Title: Determination of the intramammary dose of benzylpenicillin required to maintain an adequate concentration in the milk to inhibit Gram-positive bacteria in the clinically normal udder for 24 hr
Author: Abbeloos, Elke; Pyörälä, Satu; Rajala-Schultz, Päivi; Myllys, Vesa
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Production Animal Medicine
Date: 2018-10
Language: eng
Number of pages: 8
Belongs to series: Journal of Veterinary Pharmacology and Therapeutics
ISSN: 0140-7783
URI: http://hdl.handle.net/10138/323998
Abstract: The aim of this study was to determine the intramammary dose of benzylpenicillin required to maintain a concentration in the milk above the MIC for the Gram-positive bacteria that cause mastitis. The product used in this study was a commercially available procaine benzylpenicillin in an oily suspension with micronized particles. Three dose levels were used: 200,000, 300,000, and 600,000IU. Concentrations of benzylpenicillin in cow milk and plasma were determined after a single intramammary dose was administered into one quarter of each of the five cows in each treatment group. Samples were analyzed using an HPLC-MS/MS method, which was validated during the study. Concentrations in the milk were well above the MIC for the target pathogens for all doses tested. There was a linear dose-dependent increase in the mean AUCs of benzylpenicillin concentrations in plasma and milk. At the first milking, 12hr after dosing, there was a significant difference between the mean milk benzylpenicillin concentrations in cows treated with a dose of 600,000IU, and those treated with 200,000 or 300,000IU. Although this study shows a linear relationship between the dose of procaine benzylpenicillin administered and the concentration in the milk in the healthy udder, it would be useful to conduct studies on cows with mastitis to define the optimum dose and duration of intramammary treatment with benzylpenicillin.
Subject: -lactam antibiotics
clinical mastitis
Gram-positive bacteria
intramammary
penicillin
BOVINE MASTITIS
STAPHYLOCOCCUS-AUREUS
STREPTOCOCCUS-UBERIS
DAIRY HERDS
COWS
PENICILLIN
THERAPY
QUANTIFICATION
SUSCEPTIBILITY
PREVALENCE
413 Veterinary science
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