Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity

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Cantuti-Castelvetri , L , Ojha , R , Pedro , L D , Djannatian , M , Franz , J , Kuivanen , S , van der Meer , F , Kallio , K , Kaya , T , Anastasina , M , Smura , T , Levanov , L , Szirovicza , L , Tobi , A , Kallio-Kokko , H , Österlund , P , Joensuu , M , Meunier , F A , Butcher , S J , Winkler , M S , Mollenhauer , B , Helenius , A , Gokce , O , Teesalu , T , Hepojoki , J , Vapalahti , O , Stadelmann , C , Balistreri , G & Simons , M 2020 , ' Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity ' , Science , vol. 370 , no. 6518 , pp. 856-+ . https://doi.org/10.1126/science.abd2985

Title: Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Author: Cantuti-Castelvetri, Ludovico; Ojha, Ravi; Pedro, Liliana D.; Djannatian, Minou; Franz, Jonas; Kuivanen, Suvi; van der Meer, Franziska; Kallio, Katri; Kaya, Tugberk; Anastasina, Maria; Smura, Teemu; Levanov, Lev; Szirovicza, Leonora; Tobi, Allan; Kallio-Kokko, Hannimari; Österlund, Pamela; Joensuu, Merja; Meunier, Frederic A.; Butcher, Sarah J.; Winkler, Martin Sebastian; Mollenhauer, Brit; Helenius, Ari; Gokce, Ozgun; Teesalu, Tambet; Hepojoki, Jussi; Vapalahti, Olli; Stadelmann, Christine; Balistreri, Giuseppe; Simons, Mikael
Contributor: University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Department of Virology
University of Helsinki, Molecular and Integrative Biosciences Research Programme
University of Helsinki, University of Zurich
University of Helsinki, Helsinki One Health (HOH)
University of Helsinki, Molecular and Integrative Biosciences Research Programme
Date: 2020-11-13
Language: eng
Number of pages: 40
Belongs to series: Science
ISSN: 0036-8075
URI: http://hdl.handle.net/10138/324014
Abstract: The causative agent of coronavirus disease 2019 (COVID-19) is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For many viruses, tissue tropism is determined by the availability of virus receptors and entry cofactors on the surface of host cells. In this study, we found that neuropilin-1 (NRP1), known to bind furin-cleaved substrates, significantly potentiates SARS-CoV-2 infectivity, an effect blocked by a monoclonal blocking antibody against NRP1. A SARS-CoV-2 mutant with an altered furin cleavage site did not depend on NRP1 for infectivity. Pathological analysis of olfactory epithelium obtained from human COVID-19 autopsies revealed that SARS-CoV-2 infected NRP1-positive cells facing the nasal cavity. Our data provide insight into SARS-CoV-2 cell infectivity and define a potential target for antiviral intervention.
Subject: VIRUS
PROTEIN
SPIKE
11832 Microbiology and virology
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