Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

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Zhang , X , Viitala , T , Harjumäki , R , Kartal-Hodzic , A , Valle-Delgado , J J & Österberg , M 2021 , ' Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance ' , Journal of Colloid and Interface Science , vol. 584 , pp. 310-319 . https://doi.org/10.1016/j.jcis.2020.09.080

Title: Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance
Author: Zhang, Xue; Viitala, Tapani; Harjumäki, Riina; Kartal-Hodzic, Alma; Valle-Delgado, Juan Jose; Österberg, Monika
Contributor: University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, Tissue engineering for drug research
University of Helsinki, Research Services
Date: 2021-02-15
Language: eng
Number of pages: 10
Belongs to series: Journal of Colloid and Interface Science
ISSN: 0021-9797
URI: http://hdl.handle.net/10138/324199
Abstract: The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. Plant based, chemically unmodified cellulose nanofibrils (CNF) hydrogel is a natural, abundant, and biocompatible material that has attracted great attention for biomedical applications, in particular for threedimensional cell cultures. However, the mechanisms of cell-CNF interactions and factors that affect these interactions are not yet fully understood. In this work, multi-parametric surface plasmon resonance (SPR) was used to study how the adsorption of human hepatocellular carcinoma (HepG2) cells on CNF films is affected by the different proteins and components of the cell medium. Both human recombinant laminin 521 (LN-521, a natural protein of the extracellular matrix) and poly -L-lysine (PLL) adsorbed on CNF films and enhanced the attachment of HepG2 cells. Cell medium components (glucose and amino acids) and serum proteins (fetal bovine serum, FBS) also adsorbed on both bare CNF and on protein-coated CNF substrates. However, the adsorption of FBS hindered the attachment of HepG2 cells to LN-521and PLLcoated CNF substrates, suggesting that serum proteins blocked the formation of laminin-integrin bonds and decreased favorable PLL-cell electrostatic interactions. This work sheds light on the effect of different factors on cell attachment to CNF, paving the way for the utilization and optimization of CNF-based materials for different tissue engineering applications. (C) 2020 The Authors. Published by Elsevier Inc.
Subject: Cellulose nanofiils
Cell adsorption
Proteins
Surface plasmon resonance
Interactions
REAL-TIME
ADSORPTION
ADHESION
FILMS
PROTEIN
MODEL
BINDING
GROWTH
SERUM
LINE
318 Medical biotechnology
116 Chemical sciences
317 Pharmacy
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