Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells

Show full item record



Permalink

http://hdl.handle.net/10138/324487

Citation

Endzelins , E , Abols , A , Buss , A , Zandberga , E , Palviainen , M J , Siljander , P R-M & Line , A 2018 , ' Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells ' , Anticancer Research , vol. 38 , no. 9 , pp. 5139-5147 . https://doi.org/10.21873/anticanres.12836

Title: Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells
Author: Endzelins, Edgars; Abols, Arturs; Buss, Arturs; Zandberga, Elina; Palviainen, Mari Johanna; Siljander, Pia Riitta-Maria; Line, Aija
Other contributor: University of Helsinki, Extracellular Vesicles
University of Helsinki, Extracellular Vesicles



Date: 2018-09
Language: eng
Number of pages: 9
Belongs to series: Anticancer Research
ISSN: 0250-7005
DOI: https://doi.org/10.21873/anticanres.12836
URI: http://hdl.handle.net/10138/324487
Abstract: Background/Aim: Tumor-secreted extracellular vesicles (EVs) play an important role as mediators of intercellular communication. Hypoxia is a common feature of solid tumors frequently associated with an aggressive clinical behavior. This study aimed to gain a deeper understanding into the functions of EVs in intercellular communication between primary and metastatic cancer cells under hypoxic conditions. Materials and Methods: EVs were isolated from two isogenic colorectal cancer (CRC) cell lines SW480 and SW620 cultured under normoxic and hypoxic conditions. Their uptake and effects in SW480 and SW620 cells were studied using EV uptake, proliferation, spheroid-formation, wound healing and invasion assays. Results: Our data showed that hypoxia enhanced the release of EVs from CRC cells in a Hypoxia Induced Factor (HIF)-1-dependent manner. Hypoxic EVs were taken up by CRC cells more efficiently than normoxic EVs. Hypoxic EVs stimulated motility, invasiveness and sternness of primary tumour-derived SW480 cells, whereas they had a little effect on metastasis-derived SW620 cells. Conclusion: Hypoxic colorectal cancer-derived EVs confer aggressiveness and invasiveness to hypoxia-naive cancer cells.
Subject: 317 Pharmacy
Extracellular vesicles
colorectal cancer
hypoxia
PROMOTE ANGIOGENESIS
CARCINOMA-CELLS
EXOSOMES
EXPRESSION
STEMNESS
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Extracellular_V ... etastatic_Cancer_Cells.pdf 381.3Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record