Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1

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Khatun , M , Arffman , R K , Lavogina , D , Kangasniemi , M , Laru , J , Ahtikoski , A , Lehtonen , S , Paulson , M , Hirschberg , A L , Salumets , A , Andersson , L C & Piltonen , T T 2020 , ' Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1 ' , Biology of Reproduction , vol. 102 , no. 2 , pp. 306-315 . https://doi.org/10.1093/biolre/ioz180

Title: Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1
Author: Khatun, Masuma; Arffman, Riikka K.; Lavogina, Darja; Kangasniemi, Marika; Laru, Johanna; Ahtikoski, Anne; Lehtonen, Siri; Paulson, Mariana; Hirschberg, Angelica Linden; Salumets, Andres; Andersson, Leif C.; Piltonen, Terhi T.
Contributor organization: HUS Gynecology and Obstetrics
Clinicum
Department of Obstetrics and Gynecology
Medicum
Department of Pathology
University of Helsinki
Date: 2020-02
Language: eng
Number of pages: 10
Belongs to series: Biology of Reproduction
ISSN: 0006-3363
DOI: https://doi.org/10.1093/biolre/ioz180
URI: http://hdl.handle.net/10138/325184
Abstract: Stanniocalcin-1 (STC-1) is a pro-survival factor that protects tissues against stressors, such as hypoxia and inflammation. STC-1 is co-expressed with the endometrial receptivity markers, and recently endometrial STC-1 was reported to be dysregulated in endometriosis, a condition linked with endometrial progesterone resistance and inflammation. These features are also common in the endometrium in women with polycystic ovary syndrome (PCOS), the most common endocrine disorder in women. Given that women with PCOS present with subfertility, pregnancy complications, and increased risk for endometrial cancer, we investigated endometrial STC-1 expression in affected women. Endometrial biopsy samples were obtained from women with PCOS and controls, including samples from overweight/obese women with PCOS before and after a 3-month lifestyle intervention. A total of 98 PCOS and 85 control samples were used in immunohistochemistry, reverse-transcription polymerase chain reaction, or in vitro cell culture. STC-1 expression was analyzed at different cycle phases and in endometrial stromal cells (eSCs) after steroid hormone exposure. The eSCs were also challenged with 8-bromo-cAMP and hypoxia for STC-1 expression. The findings indicate that STC-1 expression is not steroid hormone mediated although secretory-phase STC-1 expression was blunted in PCOS. Lower expression seems to be related to attenuated STC-1 response to stressors in PCOS eSCs, shown as downregulation of protein kinase A activity. The 3-month lifestyle intervention did not restore STC-1 expression in PCOS endometrium. More studies are warranted to further elucidate the mechanisms behind the altered endometrial STC-1 expression and rescue mechanism in the PCOS endometrium. Summary sentence Endometrial expression of STC-1 in the secretory phase is blunted in women with PCOS, suggesting impaired protection against stress.
Subject: stanniocalcin-1
polycystic ovary syndrome
human endometrium
primary cell culture
endometrial stromal cells
hypoxia
stress
lifestyle intervention
RENAL ISCHEMIA/REPERFUSION INJURY
GENE-EXPRESSION
LIFE-STYLE
PREGNANCY COMPLICATIONS
STROMAL FIBROBLASTS
EPITHELIAL-CELLS
SYNDROME PCOS
METFORMIN
RECEPTOR
CANCER
3123 Gynaecology and paediatrics
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by_nc
Usage restriction: openAccess
Self-archived version: publishedVersion


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