A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts

Näytä kaikki kuvailutiedot




Hayes , A , Nguyen , D , Andersson , M , Anton , A , Bailly , J-L , Beard , S , Benschop , K S M , Berginc , N , Blomqvist , S , Cunningham , E , Davis , D , Dembinski , J L , Diedrich , S , Dudman , S G , Dyrdak , R , Eltringham , G J A , Gonzales-Goggia , S , Gunson , R , Howson-Wells , H C , Jääskeläinen , A J , Lopez-Labrador , F X , Maier , M , Majumdar , M , Midgley , S , Mirand , A , Morley , U , Nordbo , S A , Oikarinen , S , Osman , H , Papa , A , Pellegrinelli , L , Piralla , A , Rabella , N , Richter , J , Smith , M , Strand , A S , Templeton , K , Vipond , B , Vuorinen , T , Williams , C , Wollants , E , Zakikhany , K , Fischer , T K , Harvala , H & Simmonds , P 2020 , ' A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts ' , Journal of Medical Virology , vol. 92 , no. 8 , pp. 1065-1074 . https://doi.org/10.1002/jmv.25659

Julkaisun nimi: A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts
Tekijä: Hayes, A.; Nguyen, D.; Andersson, M.; Anton, A.; Bailly, J-L; Beard, S.; Benschop, K. S. M.; Berginc, N.; Blomqvist, S.; Cunningham, E.; Davis, D.; Dembinski, J. L.; Diedrich, S.; Dudman, S. G.; Dyrdak, R.; Eltringham, G. J. A.; Gonzales-Goggia, S.; Gunson, R.; Howson-Wells, H. C.; Jääskeläinen, A. J.; Lopez-Labrador, F. X.; Maier, M.; Majumdar, M.; Midgley, S.; Mirand, A.; Morley, U.; Nordbo, S. A.; Oikarinen, S.; Osman, H.; Papa, A.; Pellegrinelli, L.; Piralla, A.; Rabella, N.; Richter, J.; Smith, M.; Strand, A. Söderlund; Templeton, K.; Vipond, B.; Vuorinen, T.; Williams, C.; Wollants, E.; Zakikhany, K.; Fischer, T. K.; Harvala, H.; Simmonds, P.
Tekijän organisaatio: Medicum
Staff Services
Viral Zoonosis Research Unit
Department of Virology
Päiväys: 2020-08
Kieli: eng
Sivumäärä: 10
Kuuluu julkaisusarjaan: Journal of Medical Virology
ISSN: 0146-6615
DOI-tunniste: https://doi.org/10.1002/jmv.25659
URI: http://hdl.handle.net/10138/325219
Tiivistelmä: Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 mu L) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 mu L) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 x 10(-5)). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests.
Avainsanat: enterovirus
enterovirus A71
RNA transcripts
11832 Microbiology and virology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: acceptedVersion


Latausmäärä yhteensä: Ladataan...

Tiedosto(t) Koko Formaatti Näytä
jmv.25659.pdf 2.250MB PDF Avaa tiedosto
A_European_mult ... _using_RNA_transcripts.pdf 3.553MB PDF Avaa tiedosto

Viite kuuluu kokoelmiin:

Näytä kaikki kuvailutiedot