A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts

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dc.contributor.author Hayes, A.
dc.contributor.author Nguyen, D.
dc.contributor.author Andersson, M.
dc.contributor.author Anton, A.
dc.contributor.author Bailly, J-L
dc.contributor.author Beard, S.
dc.contributor.author Benschop, K. S. M.
dc.contributor.author Berginc, N.
dc.contributor.author Blomqvist, S.
dc.contributor.author Cunningham, E.
dc.contributor.author Davis, D.
dc.contributor.author Dembinski, J. L.
dc.contributor.author Diedrich, S.
dc.contributor.author Dudman, S. G.
dc.contributor.author Dyrdak, R.
dc.contributor.author Eltringham, G. J. A.
dc.contributor.author Gonzales-Goggia, S.
dc.contributor.author Gunson, R.
dc.contributor.author Howson-Wells, H. C.
dc.contributor.author Jääskeläinen, A. J.
dc.contributor.author Lopez-Labrador, F. X.
dc.contributor.author Maier, M.
dc.contributor.author Majumdar, M.
dc.contributor.author Midgley, S.
dc.contributor.author Mirand, A.
dc.contributor.author Morley, U.
dc.contributor.author Nordbo, S. A.
dc.contributor.author Oikarinen, S.
dc.contributor.author Osman, H.
dc.contributor.author Papa, A.
dc.contributor.author Pellegrinelli, L.
dc.contributor.author Piralla, A.
dc.contributor.author Rabella, N.
dc.contributor.author Richter, J.
dc.contributor.author Smith, M.
dc.contributor.author Strand, A. Söderlund
dc.contributor.author Templeton, K.
dc.contributor.author Vipond, B.
dc.contributor.author Vuorinen, T.
dc.contributor.author Williams, C.
dc.contributor.author Wollants, E.
dc.contributor.author Zakikhany, K.
dc.contributor.author Fischer, T. K.
dc.contributor.author Harvala, H.
dc.contributor.author Simmonds, P.
dc.date.accessioned 2021-01-26T10:12:01Z
dc.date.available 2021-01-26T10:12:01Z
dc.date.issued 2020-08
dc.identifier.citation Hayes , A , Nguyen , D , Andersson , M , Anton , A , Bailly , J-L , Beard , S , Benschop , K S M , Berginc , N , Blomqvist , S , Cunningham , E , Davis , D , Dembinski , J L , Diedrich , S , Dudman , S G , Dyrdak , R , Eltringham , G J A , Gonzales-Goggia , S , Gunson , R , Howson-Wells , H C , Jääskeläinen , A J , Lopez-Labrador , F X , Maier , M , Majumdar , M , Midgley , S , Mirand , A , Morley , U , Nordbo , S A , Oikarinen , S , Osman , H , Papa , A , Pellegrinelli , L , Piralla , A , Rabella , N , Richter , J , Smith , M , Strand , A S , Templeton , K , Vipond , B , Vuorinen , T , Williams , C , Wollants , E , Zakikhany , K , Fischer , T K , Harvala , H & Simmonds , P 2020 , ' A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts ' , Journal of Medical Virology , vol. 92 , no. 8 , pp. 1065-1074 . https://doi.org/10.1002/jmv.25659
dc.identifier.other PURE: 159553151
dc.identifier.other PURE UUID: c7062147-c7a4-42e7-99fd-643aef325bda
dc.identifier.other WOS: 000507666000001
dc.identifier.uri http://hdl.handle.net/10138/325219
dc.description.abstract Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 mu L) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 mu L) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 x 10(-5)). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Journal of Medical Virology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject enterovirus
dc.subject enterovirus A71
dc.subject parechovirus
dc.subject PCR
dc.subject RNA transcripts
dc.subject CEREBROSPINAL-FLUID
dc.subject CLINICAL-SAMPLES
dc.subject INFECTIONS
dc.subject EPIDEMIOLOGY
dc.subject ASSOCIATION
dc.subject RHINOVIRUS
dc.subject DIVERSITY
dc.subject CHILDREN
dc.subject PANEL
dc.subject 11832 Microbiology and virology
dc.title A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization HUSLAB
dc.contributor.organization Staff Services
dc.contributor.organization Viral Zoonosis Research Unit
dc.contributor.organization Department of Virology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1002/jmv.25659
dc.relation.issn 0146-6615
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion
dc.type.version publishedVersion

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