An inducible genome editing system for plants

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Wang, X, Ye, L, Lyu, M, Ursache, R, Löytynoja, A & Mähönen, A P 2020, ' An inducible genome editing system for plants ', Nature plants, vol. 6, no. 7, pp. 766–772 . https://doi.org/10.1038/s41477-020-0695-2

Title: An inducible genome editing system for plants
Author: Wang, Xin; Ye, Lingling; Lyu, Munan; Ursache, Robertas; Löytynoja, Ari; Mähönen, Ari Pekka
Contributor organization: Helsinki Institute of Life Science HiLIFE
Institute of Biotechnology
Organismal and Evolutionary Biology Research Programme
Viikki Plant Science Centre (ViPS)
Ari Pekka Löytynoja / Principal Investigator
Date: 2020-07
Language: eng
Number of pages: 24
Belongs to series: Nature plants
ISSN: 2055-026X
DOI: https://doi.org/10.1038/s41477-020-0695-2
URI: http://hdl.handle.net/10138/325526
Abstract: Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type-specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a new tool with which target genes can efficiently and conditionally be knocked out by genome editing at any developmental stage. Target genes can also be knocked out in a cell-type-specific manner. Our tool is easy to construct and will be particularly useful for studying genes having null alleles that are non-viable or show pleiotropic developmental defects.
Subject: STEM-CELL MAINTENANCE
AUXIN TRANSPORT
GENE-EXPRESSION
DNA-DAMAGE
ARABIDOPSIS
GNOM
GENERATION
PROTEINS
DELETION
VECTORS
11831 Plant biology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


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