Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics

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Shetty , S A , Boeren , S , Bui , T P N , Smidt , H & de Vos , W M 2020 , ' Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics ' , Environmental Microbiology , vol. 22 , no. 11 , pp. 4863-4875 . https://doi.org/10.1111/1462-2920.15269

Title: Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics
Author: Shetty, Sudarshan A.; Boeren, Sjef; Bui, Thi P. N.; Smidt, Hauke; de Vos, Willem M.
Contributor: University of Helsinki, Department of Bacteriology and Immunology
Date: 2020-11
Language: eng
Number of pages: 13
Belongs to series: Environmental Microbiology
ISSN: 1462-2912
URI: http://hdl.handle.net/10138/325641
Abstract: Thed- andl-forms of lactate are important fermentation metabolites produced by intestinal bacteria but are found to negatively affect mucosal barrier function and human health. Both enantiomers of lactate can be converted with acetate into the presumed beneficial butyrate by a phylogenetically related group of anaerobes, includingAnaerobutyricumandAnaerostipesspp. This is a low energy yielding process with a partially unknown pathway inAnaerobutyricumandAnaerostipesspp. and hence, we sought to address this via a comparative genomics, proteomics and physiology approach. We compared growth ofAnaerobutyricum soehngeniion lactate with that on sucrose and sorbitol. Comparative proteomics revealed complete pathway of butyrate formation from sucrose, sorbitol and lactate. Notably, a gene cluster,lctABCDEFwas abundantly expressed when grown on lactate. This gene cluster encodes a lactate dehydrogenase (lctD), electron transport proteins A and B (lctCB), nickel-dependent racemase (lctE), lactate permease (lctF) and short-chain acyl-CoA dehydrogenase (lctG). Investigation of available genomes of intestinal bacteria revealed this new gene cluster to be highly conserved in onlyAnaerobutyricumandAnaerostipesspp. Present study demonstrates thatA. soehngeniiand several relatedAnaerobutyricumandAnaerostipesspp. are highly adapted for a lifestyle involving lactate plus acetate utilization in the human intestinal tract.
Subject: SP NOV.
ANAEROSTIPES-RHAMNOSIVORANS
PROTEIN COMPLEXES
COMB. NOV.
GEN. NOV.
BACTERIUM
IDENTIFICATION
RECLASSIFICATION
PROPIONATE
MICROBIOTA
3111 Biomedicine
11832 Microbiology and virology
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