A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

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Barmaki , S , Obermaier , D , Kankuri , E , Vuola , J , Franssila , S & jokinen , V 2020 , ' A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture ' , Micromachines , vol. 11 , no. 11 , 979 . https://doi.org/10.3390/mi11110979

Title: A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture
Author: Barmaki, Samineh; Obermaier, Daniela; Kankuri, Esko; Vuola, Jyrki; Franssila, Sami; jokinen, Ville
Contributor organization: HUMI - Human Microbiome Research
Medicum
Department of Pharmacology
Faculty of Medicine
University of Helsinki
Esko Markus Kankuri / Principal Investigator
HUS Musculoskeletal and Plastic Surgery
Plastiikkakirurgian yksikkö
University Management
Helsinki University Hospital Area
Date: 2020-11
Language: eng
Number of pages: 14
Belongs to series: Micromachines
ISSN: 2072-666X
DOI: https://doi.org/10.3390/mi11110979
URI: http://hdl.handle.net/10138/325722
Abstract: A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales.
Subject: DEVICES
DIFFUSION
OXYGEN GRADIENTS
PLATFORM
cell culture
gasotransmitter
hypoxia
microenvironment
microfluidic chip
nitric oxide
oxygen depletion
sodium nitroprusside
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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