A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

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dc.contributor.author Barmaki, Samineh
dc.contributor.author Obermaier, Daniela
dc.contributor.author Kankuri, Esko
dc.contributor.author Vuola, Jyrki
dc.contributor.author Franssila, Sami
dc.contributor.author jokinen, Ville
dc.date.accessioned 2021-02-03T05:47:01Z
dc.date.available 2021-02-03T05:47:01Z
dc.date.issued 2020-11
dc.identifier.citation Barmaki , S , Obermaier , D , Kankuri , E , Vuola , J , Franssila , S & jokinen , V 2020 , ' A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture ' , Micromachines , vol. 11 , no. 11 , 979 . https://doi.org/10.3390/mi11110979
dc.identifier.other PURE: 151177773
dc.identifier.other PURE UUID: 53644198-1da8-44cc-a46b-902b61dc32f4
dc.identifier.other WOS: 000593354000001
dc.identifier.other ORCID: /0000-0003-1831-1889/work/88209943
dc.identifier.uri http://hdl.handle.net/10138/325722
dc.description.abstract A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Micromachines
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject DEVICES
dc.subject DIFFUSION
dc.subject OXYGEN GRADIENTS
dc.subject PLATFORM
dc.subject cell culture
dc.subject gasotransmitter
dc.subject hypoxia
dc.subject microenvironment
dc.subject microfluidic chip
dc.subject nitric oxide
dc.subject oxygen depletion
dc.subject sodium nitroprusside
dc.subject 3111 Biomedicine
dc.title A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture en
dc.type Article
dc.contributor.organization HUMI - Human Microbiome Research
dc.contributor.organization Medicum
dc.contributor.organization Department of Pharmacology
dc.contributor.organization Faculty of Medicine
dc.contributor.organization University of Helsinki
dc.contributor.organization Esko Markus Kankuri / Principal Investigator
dc.contributor.organization HUS Musculoskeletal and Plastic Surgery
dc.contributor.organization Plastiikkakirurgian yksikkö
dc.contributor.organization University Management
dc.contributor.organization Helsinki University Hospital Area
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/mi11110979
dc.relation.issn 2072-666X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.type.version publishedVersion

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