Phage lysin that specifically eliminates Clostridium botulinum Group I cells

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Zhang , Z , Lahti , M , Douillard , F P , Korkeala , H & Lindstrom , M 2020 , ' Phage lysin that specifically eliminates Clostridium botulinum Group I cells ' , Scientific Reports , vol. 10 , no. 1 , 21571 . https://doi.org/10.1038/s41598-020-78622-6

Title: Phage lysin that specifically eliminates Clostridium botulinum Group I cells
Author: Zhang, Zhen; Lahti, Meeri; Douillard, Francois P.; Korkeala, Hannu; Lindstrom, Miia
Contributor: University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Helsinki One Health (HOH)
University of Helsinki, Food Hygiene and Environmental Health
Date: 2020-12-09
Language: eng
Number of pages: 10
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/325891
Abstract: Clostridium botulinum poses a serious threat to food safety and public health by producing potent neurotoxin during its vegetative growth and causing life-threatening neuroparalysis, botulism. While high temperature can be utilized to eliminate C. botulinum spores and the neurotoxin, non-thermal elimination of newly germinated C. botulinum cells before onset of toxin production could provide an alternative or additional factor controlling the risk of botulism in some applications. Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the concentration of 5 mu M, but shows little or no lytic activity against C. botulinum Group II or III or other Firmicutes strains. CBO1751 is active at pH from 6.5 to 10.5. The lytic activity of CBO1751 is tolerant to NaCl (200 mM), but highly susceptible to divalent cations Ca2+ and Mg2+ (50 mM). CBO1751 readily and effectively eliminates C. botulinum during spore germination, an early stage preceding vegetative growth and neurotoxin production. This is the first report of an antimicrobial lysin against C. botulinum, presenting high potential for developing a novel antibotulinal agent for non-thermal applications in food and agricultural industries.
Subject: BACTERIOPHAGE ENDOLYSINS
MUREIN HYDROLASE
GENOMIC SEQUENCE
PERFRINGENS
EXPRESSION
VIRULENT
BIOLOGY
DOMAIN
11832 Microbiology and virology
416 Food Science
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