Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection

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Joraholmen , M W , Johannessen , M , Gravningen , K , Puolakkainen , M , Acharya , G , Basnet , P & Skalko-Basnet , N 2020 , ' Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection ' , Pharmaceutics , vol. 12 , no. 12 , 1203 . https://doi.org/10.3390/pharmaceutics12121203

Title: Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection
Author: Joraholmen, May Wenche; Johannessen, Mona; Gravningen, Kirsten; Puolakkainen, Mirja; Acharya, Ganesh; Basnet, Purusotam; Skalko-Basnet, Natasa
Contributor organization: HUSLAB
Mirja Puolakkainen / Principal Investigator
Department of Virology
University of Helsinki
Helsinki University Hospital Area
Date: 2020-12
Language: eng
Number of pages: 14
Belongs to series: Pharmaceutics
ISSN: 1999-4923
DOI: https://doi.org/10.3390/pharmaceutics12121203
URI: http://hdl.handle.net/10138/326119
Abstract: Chlamydia trachomatis is the most common cause of bacterial sexually transmitted infections and causes serious reproductive tract complications among women. The limitations of existing oral antibiotics and treatment of antimicrobial resistance require alternative treatment options. We are proposing, for the first time, the natural polyphenol resveratrol (RES) in an advanced delivery system comprising liposomes incorporated in chitosan hydrogel, for the localized treatment of C. trachomatis infection. Both free RES and RES liposomes-in-hydrogel inhibited the propagation of C. trachomatis in a concentration-dependent manner, assessed by the commonly used in vitro model comprising McCoy cells. However, for lower concentrations, the anti-chlamydial effect of RES was enhanced when incorporated into a liposomes-in-hydrogel delivery system, with inhibition of 78% and 94% for 1.5 and 3 mu g/mL RES, respectively for RES liposomes-in-hydrogel, compared to 43% and 72%, respectively, for free RES. Furthermore, RES liposomes-in-hydrogel exhibited strong anti-inflammatory activity in vitro, in a concentration-dependent inhibition of nitric oxide production in the LPS-induced macrophages (RAW 264.7). The combination of a natural substance exhibiting multi-targeted pharmacological properties, and a delivery system that provides enhanced activity as well as applicability for vaginal administration, could be a promising option for the localized treatment of C. trachomatis infection.
Subject: resveratrol
chitosan
liposomes
hydrogel
Chlamydia trachomatis
antimicrobial
vaginal therapy
THERAPY
STABILITY
317 Pharmacy
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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