Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection

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dc.contributor.author Joraholmen, May Wenche
dc.contributor.author Johannessen, Mona
dc.contributor.author Gravningen, Kirsten
dc.contributor.author Puolakkainen, Mirja
dc.contributor.author Acharya, Ganesh
dc.contributor.author Basnet, Purusotam
dc.contributor.author Skalko-Basnet, Natasa
dc.date.accessioned 2021-02-08T10:31:01Z
dc.date.available 2021-02-08T10:31:01Z
dc.date.issued 2020-12
dc.identifier.citation Joraholmen , M W , Johannessen , M , Gravningen , K , Puolakkainen , M , Acharya , G , Basnet , P & Skalko-Basnet , N 2020 , ' Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection ' , Pharmaceutics , vol. 12 , no. 12 , 1203 . https://doi.org/10.3390/pharmaceutics12121203
dc.identifier.other PURE: 160102293
dc.identifier.other PURE UUID: 9f5f133e-c40c-4808-a8d7-0b91fd20878d
dc.identifier.other WOS: 000603090500001
dc.identifier.other ORCID: /0000-0001-7413-6793/work/88675537
dc.identifier.uri http://hdl.handle.net/10138/326119
dc.description.abstract Chlamydia trachomatis is the most common cause of bacterial sexually transmitted infections and causes serious reproductive tract complications among women. The limitations of existing oral antibiotics and treatment of antimicrobial resistance require alternative treatment options. We are proposing, for the first time, the natural polyphenol resveratrol (RES) in an advanced delivery system comprising liposomes incorporated in chitosan hydrogel, for the localized treatment of C. trachomatis infection. Both free RES and RES liposomes-in-hydrogel inhibited the propagation of C. trachomatis in a concentration-dependent manner, assessed by the commonly used in vitro model comprising McCoy cells. However, for lower concentrations, the anti-chlamydial effect of RES was enhanced when incorporated into a liposomes-in-hydrogel delivery system, with inhibition of 78% and 94% for 1.5 and 3 mu g/mL RES, respectively for RES liposomes-in-hydrogel, compared to 43% and 72%, respectively, for free RES. Furthermore, RES liposomes-in-hydrogel exhibited strong anti-inflammatory activity in vitro, in a concentration-dependent inhibition of nitric oxide production in the LPS-induced macrophages (RAW 264.7). The combination of a natural substance exhibiting multi-targeted pharmacological properties, and a delivery system that provides enhanced activity as well as applicability for vaginal administration, could be a promising option for the localized treatment of C. trachomatis infection. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Pharmaceutics
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject resveratrol
dc.subject chitosan
dc.subject liposomes
dc.subject hydrogel
dc.subject Chlamydia trachomatis
dc.subject antimicrobial
dc.subject vaginal therapy
dc.subject THERAPY
dc.subject STABILITY
dc.subject 317 Pharmacy
dc.subject 11832 Microbiology and virology
dc.title Liposomes-In-Hydrogel Delivery System Enhances the Potential of Resveratrol in Combating Vaginal Chlamydia Infection en
dc.type Article
dc.contributor.organization HUSLAB
dc.contributor.organization Mirja Puolakkainen / Principal Investigator
dc.contributor.organization Department of Virology
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki University Hospital Area
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/pharmaceutics12121203
dc.relation.issn 1999-4923
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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