Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species

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http://hdl.handle.net/10138/326147

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Cui , F , Wu , H , Safronov , O , Zhang , P , Kumar , R , Kollist , H , Salojärvi , J T , Panstruga , R & Overmyer , K L 2018 , ' Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species ' , Plant, Cell and Environment , vol. 41 , no. 4 , pp. 782-796 . https://doi.org/10.1111/pce.13144

Julkaisun nimi: Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species
Tekijä: Cui, Fuqiang; Wu, Hongpo; Safronov, Omid; Zhang, Panpan; Kumar, Rajeev; Kollist, Hannes; Salojärvi, Jarkko Tapani; Panstruga, Ralph; Overmyer, Kirk Loren
Tekijän organisaatio: Plant-Fungal Interactions Group
Plant Biology
Biosciences
Bioinformatics for Molecular Biology and Genomics (BMBG)
Plant ROS-Signalling
Organismal and Evolutionary Biology Research Programme
Doctoral Programme in Plant Sciences
Viikki Plant Science Centre (ViPS)
Päiväys: 2018-04
Kieli: eng
Sivumäärä: 15
Kuuluu julkaisusarjaan: Plant, Cell and Environment
ISSN: 1365-3040
DOI-tunniste: https://doi.org/10.1111/pce.13144
URI: http://hdl.handle.net/10138/326147
Tiivistelmä: The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways.
Avainsanat: 1183 Plant biology, microbiology, virology
abiotic stress
immunity
ozone
ROS signalling
unequal genetic redundancy
RESPONSIVE GENE-EXPRESSION
POWDERY MILDEW
CELL-DEATH
SALICYLIC-ACID
DISEASE RESISTANCE
NONHOST RESISTANCE
OZONE SENSITIVITY
ANTIFUNGAL DEFENSE
SIGNALING PATHWAYS
STOMATAL RESPONSE
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: unspecified
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: acceptedVersion


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