Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species

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dc.contributor.author Cui, Fuqiang
dc.contributor.author Wu, Hongpo
dc.contributor.author Safronov, Omid
dc.contributor.author Zhang, Panpan
dc.contributor.author Kumar, Rajeev
dc.contributor.author Kollist, Hannes
dc.contributor.author Salojärvi, Jarkko Tapani
dc.contributor.author Panstruga, Ralph
dc.contributor.author Overmyer, Kirk Loren
dc.date.accessioned 2021-02-08T12:49:01Z
dc.date.available 2021-02-08T12:49:01Z
dc.date.issued 2018-04
dc.identifier.citation Cui , F , Wu , H , Safronov , O , Zhang , P , Kumar , R , Kollist , H , Salojärvi , J T , Panstruga , R & Overmyer , K L 2018 , ' Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species ' , Plant, Cell and Environment , vol. 41 , no. 4 , pp. 782-796 . https://doi.org/10.1111/pce.13144
dc.identifier.other PURE: 96641809
dc.identifier.other PURE UUID: ffe4233c-508b-4cb5-83c9-f13b7b6d245a
dc.identifier.other Scopus: 85042313637
dc.identifier.other WOS: 000426870500007
dc.identifier.other ORCID: /0000-0002-7398-3453/work/44473043
dc.identifier.other ORCID: /0000-0003-2044-3258/work/44473704
dc.identifier.uri http://hdl.handle.net/10138/326147
dc.description.abstract The atmospheric pollutant ozone (O-3) is a strong oxidant that causes extracellular reactive oxygen species (ROS) formation, has significant ecological relevance, and is used here as a non-invasive ROS inducer to study plant signalling. Previous genetic screens identified several mutants exhibiting enhanced O-3 sensitivity, but few with enhanced tolerance. We found that loss-of-function mutants in Arabidopsis MLO2, a gene implicated in susceptibility to powdery mildew disease, exhibit enhanced dose-dependent tolerance to O-3 and extracellular ROS, but a normal response to intracellular ROS. This phenotype is increased in a mlo2 mlo6 mlo12 triple mutant, reminiscent of the genetic redundancy of MLO genes in powdery mildew resistance. Stomatal assays revealed that enhanced O-3 tolerance in mlo2 mutants is not caused by altered stomatal conductance. We explored modulation of the mlo2-associated O-3 tolerance, powdery mildew resistance, and early senescence phenotypes by genetic epistasis analysis, involving mutants with known effects on ROS sensitivity or antifungal defence. Mining of publicly accessible microarray data suggests that these MLO proteins regulate accumulation of abiotic stress response transcripts, and transcript accumulation of MLO2 itself is O-3 responsive. In summary, our data reveal MLO2 as a novel negative regulator in plant ROS responses, which links biotic and abiotic stress response pathways. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Plant, Cell and Environment
dc.rights unspecified
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 1183 Plant biology, microbiology, virology
dc.subject abiotic stress
dc.subject immunity
dc.subject ozone
dc.subject ROS signalling
dc.subject unequal genetic redundancy
dc.subject RESPONSIVE GENE-EXPRESSION
dc.subject POWDERY MILDEW
dc.subject CELL-DEATH
dc.subject SALICYLIC-ACID
dc.subject DISEASE RESISTANCE
dc.subject NONHOST RESISTANCE
dc.subject OZONE SENSITIVITY
dc.subject ANTIFUNGAL DEFENSE
dc.subject SIGNALING PATHWAYS
dc.subject STOMATAL RESPONSE
dc.title Arabidopsis MLO2 is a negative regulator of sensitivity to extracellular reactive oxygen species en
dc.type Article
dc.contributor.organization Plant-Fungal Interactions Group
dc.contributor.organization Plant Biology
dc.contributor.organization Biosciences
dc.contributor.organization Bioinformatics for Molecular Biology and Genomics (BMBG)
dc.contributor.organization Plant ROS-Signalling
dc.contributor.organization Organismal and Evolutionary Biology Research Programme
dc.contributor.organization Doctoral Programme in Plant Sciences
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/pce.13144
dc.relation.issn 1365-3040
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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