A high-throughput approach to the culture-based estimation of plasmid transfer rates

Show full item record



Permalink

http://hdl.handle.net/10138/326228

Citation

Kneis , D , Hiltunen , T & Hess , S 2019 , ' A high-throughput approach to the culture-based estimation of plasmid transfer rates ' , Plasmid , vol. 101 , pp. 28-34 . https://doi.org/10.1016/j.plasmid.2018.12.003

Title: A high-throughput approach to the culture-based estimation of plasmid transfer rates
Author: Kneis, David; Hiltunen, Teppo; Hess, Stefanie
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2019-01
Language: eng
Number of pages: 7
Belongs to series: Plasmid
ISSN: 0147-619X
URI: http://hdl.handle.net/10138/326228
Abstract: Horizontal gene transfer is an essential component of bacterial evolution. Quantitative information on transfer rates is particularly useful to better understand and possibly predict the spread of antimicrobial resistance. A variety of methods has been proposed to estimate the rates of plasmid-mediated gene transfer all of which require substantial labor input or financial resources. A cheap but reliable method with high-throughput capabilities is yet to be developed in order to better capture the variability of plasmid transfer rates, e.g. among strains or in response to environmental cues. We explored a new approach to the culture-based estimation of plasmid transfer rates in liquid media allowing for a large number of parallel experiments. It deviates from established approaches in the fact that it exploits data on the absence/presence of transconjugant cells in the wells of a well plate observed over time. Specifically, the binary observations are compared to the probability of transconjugant detection as predicted by a dynamic model. The bulk transfer rate is found as the best-fit value of a designated model parameter. The feasibility of the approach is demonstrated on mating experiments where the RP4 plasmid is transfered from Serratia marcescens to several Escherichia coil recipients. The methods uncertainty is explored via split sampling and virtual experiments.
Subject: Horizontal gene transfer
Conjugation
Parameter estimation
Escherichia coli
Serratia marcescens
RP4 plasmid
ESCHERICHIA-COLI
ANTIBIOTIC-RESISTANCE
GENE-TRANSFER
IN-SITU
BACTERIAL
FITNESS
CONJUGATION
ENHANCEMENT
KINETICS
1183 Plant biology, microbiology, virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
plos2019.pdf 864.9Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record