Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates

Show simple item record Isakova, Elena P. Matushkina, Irina N. Popova, Tatyana N. Dergacheva, Darya Gessler, Natalya N. Klein, Olga Semenikhina, Anastasya Deryabina, Yulia La Porta, Nicola Saris, Nils-Eric L. 2021-02-09T14:12:04Z 2021-02-09T14:12:04Z 2020-01
dc.identifier.citation Isakova , E P , Matushkina , I N , Popova , T N , Dergacheva , D , Gessler , N N , Klein , O , Semenikhina , A , Deryabina , Y , La Porta , N & Saris , N-E L 2020 , ' Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates ' , Microorganisms , vol. 8 , no. 1 , 91 .
dc.identifier.other PURE: 160138596
dc.identifier.other PURE UUID: c0659015-f4ac-40e7-b097-17fe115240be
dc.identifier.other WOS: 000514650700054
dc.description.abstract In this study, we evaluated the metabolic profile of the aerobic microorganism of Endomyces magnusii with a complete respiration chain and well-developed mitochondria system during long-lasting cultivation. The yeast was grown in batches using glycerol and glucose as the sole carbon source for a week. The profile included the cellular biological and chemical parameters, which determined the redox status of the yeast cells. We studied the activities of the antioxidant systems (catalases and superoxide dismutases), glutathione system enzymes (glutathione peroxidase and reductase), aconitase, as well as the main enzymes maintaining NADPH levels in the cells (glucose-6-phosphate dehydrogenase and NADP(+)-isocitrate dehydrogenase) during aging of Endomyces magnusii on two kinds of substrates. We also investigated the dynamics of change in oxidized and reduced glutathione, conjugated dienes, and reactive oxidative species in the cells at different growth stages, including the deep stationary stages. Our results revealed a similar trend in the changes in the activity of all the enzymes tested, which increased 2-4-fold upon aging. The yeast cytosol had a very high reduced glutathione content, 22 times than that of Saccharomyces cerevisiae, and remained unchanged during growth, whereas there was a 7.5-fold increase in the reduced glutathione-to-oxidized glutathione ratio. The much higher level of reactive oxidative species was observed in the cells in the late and deep stationary phases, especially in the cells using glycerol. Cell aging of the culture grown on glycerol, which promotes active oxidative phosphorylation in the mitochondria, facilitated the functioning of powerful antioxidant systems (catalases, superoxide dismutases, and glutathione system enzymes) induced by reactive oxidative species. Moreover, it stimulated NADPH synthesis, regulating the cytosolic reduced glutathione level, which in turn determines the redox potential of the yeast cell during the early aging process. en
dc.format.extent 24
dc.language.iso eng
dc.relation.ispartof Microorganisms
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Endomyces magnusii
dc.subject mitochondria
dc.subject glutathione system
dc.subject yeast
dc.subject antioxidant enzymes
dc.subject reactive oxygen species
dc.subject aging
dc.subject LIFE-SPAN
dc.subject GLUTATHIONE
dc.subject THIOREDOXIN
dc.subject ACONITASE
dc.subject ROLES
dc.subject RESPIRATION
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Metabolic Remodeling during Long-Lasting Cultivation of the Endomyces magnusii Yeast on Oxidative and Fermentative Substrates en
dc.type Article
dc.contributor.organization Department of Food and Nutrition
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2076-2607
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
microorganisms_08_00091.pdf 3.509Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record