Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice
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Muha , V , Williamson , R , Hills , R , McNeilly , A D , McWilliams , T G , Alonso , J , Schimpl , M , Leney , A C , Heck , A J R , Sutherland , C , Read , K D , McCrimmon , R J , Brooks , S P & Van Aalten , D M F 2019 , ' Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice ' , Open biology , vol. 9 , no. 11 . https://doi.org/10.1098/rsob.190192
| Title: | Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice |
| Author: | Muha, Villo; Williamson, Ritchie; Hills, Rachel; McNeilly, A.D.; McWilliams, T.G.; Alonso, Jana; Schimpl, Marianne; Leney, Aneika C.; Heck, Albert J.R.; Sutherland, Calum; Read, Kevin D.; McCrimmon, Rory J.; Brooks, S.P.; Van Aalten, Daan M.F. |
| Contributor organization: | Department of Anatomy STEMM - Stem Cells and Metabolism Research Program |
| Date: | 2019 |
| Language: | eng |
| Number of pages: | 15 |
| Belongs to series: | Open biology |
| ISSN: | 2046-2441 |
| DOI: | https://doi.org/10.1098/rsob.190192 |
| URI: | http://hdl.handle.net/10138/326544 |
| Abstract: | O-GlcNAcylation is an abundant post-translational modification in the nervous system, linked to both neurodevelopmental and neurodegenerative disease. However, the mechanistic links between these phenotypes and site-specific O-GlcNAcylation remain largely unexplored. Here, we show that Ser517 O-GlcNAcylation of the microtubule-binding protein Collapsin Response Mediator Protein-2 (CRMP2) increases with age. By generating and characterizing a Crmp2S517A knock-in mouse model, we demonstrate that loss of O-GlcNAcylation leads to a small decrease in body weight and mild memory impairment, suggesting that Ser517 O-GlcNAcylation has a small but detectable impact on mouse physiology and cognitive function. © 2019 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
| Subject: |
Cognitive function
CRMP2 Crosstalk O-GlcNAcylation collapsin response mediator protein-2 n acetylglucosamine nerve protein signal peptide aging amino acid sequence animal C57BL mouse cell line chemistry cognition exploratory behavior female gene knock-in genetics human male memory disorder metabolism mouse point mutation protein processing short term memory Acetylglucosamine Aging Amino Acid Sequence Animals Cell Line Cognition Exploratory Behavior Female Gene Knock-In Techniques Humans Intercellular Signaling Peptides and Proteins Male Memory Disorders Memory, Short-Term Mice Mice, Inbred C57BL Nerve Tissue Proteins Point Mutation Protein Processing, Post-Translational 1182 Biochemistry, cell and molecular biology |
| Peer reviewed: | Yes |
| Rights: | cc_by |
| Usage restriction: | openAccess |
| Self-archived version: | publishedVersion |
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