Alternative oxidase confers nutritional limitation on Drosophila development

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Saari , S , Kemppainen , E , Tuomela , T , Oliveira , M T , Dufour , E & Jacobs , H T 2019 , ' Alternative oxidase confers nutritional limitation on Drosophila development ' , Journal of experimental zoology. Part A, Ecological and integrative physiology , vol. 331 , no. 6 , pp. 341-356 . https://doi.org/10.1002/jez.2274

Title: Alternative oxidase confers nutritional limitation on Drosophila development
Author: Saari, Sini; Kemppainen, Esa; Tuomela, Tero; Oliveira, M.T.; Dufour, E.; Jacobs, H.T.
Other contributor: University of Helsinki, Doctoral Programme in Integrative Life Science

Date: 2019
Language: eng
Number of pages: 16
Belongs to series: Journal of experimental zoology. Part A, Ecological and integrative physiology
ISSN: 2471-5638
DOI: https://doi.org/10.1002/jez.2274
URI: http://hdl.handle.net/10138/326580
Abstract: The mitochondrial alternative oxidase, AOX, present in most eukaryotes apart from vertebrates and insects, catalyzes the direct oxidation of ubiquinol by oxygen, by-passing the terminal proton-motive steps of the respiratory chain. Its physiological role is not fully understood, but it is proposed to buffer stresses in the respiratory chain similar to those encountered in mitochondrial diseases in humans. Previously, we found that the ubiquitous expression of AOX from Ciona intestinalis in Drosophila perturbs the development of flies cultured under low-nutrient conditions (media containing only glucose and yeast). Here we tested the effects of a wide range of nutritional supplements on Drosophila development, to gain insight into the physiological mechanism underlying this developmental failure. On low-nutrient medium, larvae contained decreased amounts of triglycerides, lactate, and pyruvate, irrespective of AOX expression. Complex food supplements, including treacle (molasses), restored normal development to AOX-expressing flies, but many individual additives did not. Inhibition of AOX by treacle extract was excluded as a mechanism, since the supplement did not alter the enzymatic activity of AOX in vitro. Furthermore, antibiotics did not influence the organismal phenotype, indicating that commensal microbes were not involved. Fractionation of treacle identified a water-soluble fraction with low solubility in ethanol, rich in lactate and tricarboxylic acid cycle intermediates, which contained the critical activity. We propose that the partial activation of AOX during metamorphosis impairs the efficient use of stored metabolites, resulting in developmental failure. © 2019 The Authors. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Published by Wiley Periodicals, Inc.
Subject: AOX
cataplerosis
mitochondria
nutrition
TCA cycle
alternative oxidase
Drosophila protein
mitochondrial protein
molasses
oxidoreductase
plant protein
animal
animal food
Ciona intestinalis
diet
Drosophila
enzymology
genetics
growth, development and aging
HEK293 cell line
human
larva
metabolism
metamorphosis
mitochondrion
transgenic animal
Animal Nutritional Physiological Phenomena
Animals
Animals, Genetically Modified
Diet
Drosophila Proteins
HEK293 Cells
Humans
Larva
Metamorphosis, Biological
Mitochondria
Mitochondrial Proteins
Molasses
Oxidoreductases
Plant Proteins
1182 Biochemistry, cell and molecular biology
413 Veterinary science
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