Front cover - Cell Membrane Wrapping: Influence of Cell Membrane Wrapping on the Cell−Porous Silicon Nanoparticle Interactions (Adv. Healthcare Mater. 17/2020)

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Fontana , F , Lindstedt , H , Correia , A , Chiaro , J , Kari , O , Ndika , J , Alenius , H , Buck , J , Sieber , S , Mäkilä , E , Salonen , J , Urtti , A , Cerullo , V , Hirvonen , J & Santos , H A 2020 , ' Front cover - Cell Membrane Wrapping: Influence of Cell Membrane Wrapping on the Cell−Porous Silicon Nanoparticle Interactions (Adv. Healthcare Mater. 17/2020) ' , Advanced Healthcare Materials , vol. 9 , no. 17 , 2070056 . https://doi.org/10.1002/adhm.202070056

Title: Front cover - Cell Membrane Wrapping: Influence of Cell Membrane Wrapping on the Cell−Porous Silicon Nanoparticle Interactions (Adv. Healthcare Mater. 17/2020)
Author: Fontana, Flavia; Lindstedt, Hanna; Correia, Alexandra; Chiaro, Jacopo; Kari, Otto; Ndika, Joseph; Alenius, Harri; Buck, Jonas; Sieber, Sandro; Mäkilä, Ermei; Salonen, Jarno; Urtti, Arto; Cerullo, Vincenzo; Hirvonen, Jouni; Santos, Hélder A.
Contributor: University of Helsinki, Nanomedicines and Biomedical Engineering
University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, Nanomedicines and Biomedical Engineering
University of Helsinki, ImmunoViroTherapy Lab
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Drug Research Program
University of Helsinki, Drug Research Program
University of Helsinki, Drug Research Program
University of Helsinki, Division of Pharmaceutical Chemistry and Technology
Date: 2020-09-09
Language: eng
Belongs to series: Advanced Healthcare Materials
ISSN: 2192-2640
URI: http://hdl.handle.net/10138/326926
Abstract: Biohybrid nanosystems represent the cutting‐edge research in biofunctionalization of micro‐ and nano‐systems. Their physicochemical properties bring along advantages in the circulation time, camouflaging from the phagocytes, and novel antigens. This is partially a result of the qualitative differences in the protein corona, and the preferential targeting and uptake in homologous cells. However, the effect of the cell membrane on the cellular endocytosis mechanisms and time has not been fully evaluated yet. Here, the effect is assessed by quantitative flow cytometry analysis on the endocytosis of hydrophilic, negatively charged porous silicon nanoparticles and on their membrane‐coated counterparts, in the presence of chemical inhibitors of different uptake pathways. Principal component analysis is used to analyze all the data and extrapolate patterns to highlight the cell‐specific differences in the endocytosis mechanisms. Furthermore, the differences in the composition of static protein corona between naked and coated particles are investigated together with how these differences affect the interaction with human macrophages. Overall, the presence of the cell membrane only influences the speed and the entity of nanoparticles association with the cells, while there is no direct effect on the endocytosis pathways, composition of protein corona, or any reduction in macrophage‐mediated uptake.
Subject: COATED NANOPARTICLES
DRUG-DELIVERY
IMPACT
VIVO PROTEIN CORONA
biohybrids
cancer cell membranes
nanoparticle uptake
nanoparticles
protein corona
3111 Biomedicine
318 Medical biotechnology
221 Nano-technology
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