Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood

Show full item record



Permalink

http://hdl.handle.net/10138/327006

Citation

Marinovic , M , Aguilar-Pontes , M V , Zhou , M , Miettinen , O K , de Vries , R , Mäkelä , M R & Hilden , S K 2018 , ' Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood ' , Fungal Genetics and Biology , vol. 112 , pp. 47-54 . https://doi.org/10.1016/j.fgb.2017.07.004

Title: Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood
Author: Marinovic, Mila; Aguilar-Pontes, Maria Victoria; Zhou, Miaomiao; Miettinen, Otto Kullervo; de Vries, Ronald; Mäkelä, Miia Riitta; Hilden, Sari Kristiina
Contributor: University of Helsinki, Fungal Genetics and Biotechnology
University of Helsinki, Finnish Museum of Natural History
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2018-03
Language: eng
Number of pages: 8
Belongs to series: Fungal Genetics and Biology
ISSN: 1087-1845
URI: http://hdl.handle.net/10138/327006
Abstract: The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.
Subject: 1184 Genetics, developmental biology, physiology
Obba rivulosa
White-rot
Lignocellulose
Solid state cultivation
Transcriptomics
CERIPORIOPSIS-SUBVERMISPORA
PHANEROCHAETE-CHRYSOSPORIUM
PHYSISPORINUS-RIVULOSUS
OXALIC-ACID
MANGANESE PEROXIDASES
OXALATE DECARBOXYLASE
DICHOMITUS-SQUALENS
SECRETOME ANALYSIS
POSTIA-PLACENTA
ENZYMES
Obba rivulosa
White-rot
Lignocellulose
Solid state cultivation
Transcriptomics
CERIPORIOPSIS-SUBVERMISPORA
PHANEROCHAETE-CHRYSOSPORIUM
PHYSISPORINUS-RIVULOSUS
OXALIC-ACID
MANGANESE PEROXIDASES
OXALATE DECARBOXYLASE
DICHOMITUS-SQUALENS
SECRETOME ANALYSIS
POSTIA-PLACENTA
ENZYMES
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Marinovic_et_al ... h_on_solid_spruce_wood.pdf 1.687Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record