Wang , A , Islam , M N , Johansen , A , Haapalainen , M , Latvala , S & Edelenbos , M 2019 , ' Pathogenic Fusarium oxysporum f. sp. cepae growing inside onion bulbs emits volatile organic compounds that correlate with the extent of infection ' , Postharvest Biology and Technology , vol. 152 , pp. 19-28 . https://doi.org/10.1016/j.postharvbio.2019.02.010
Title: | Pathogenic Fusarium oxysporum f. sp. cepae growing inside onion bulbs emits volatile organic compounds that correlate with the extent of infection |
Author: | Wang, Aimei; Islam, Md Nahidul; Johansen, Anders; Haapalainen, Minna; Latvala, Satu; Edelenbos, Merete |
Contributor organization: | Department of Agricultural Sciences |
Date: | 2019-06 |
Language: | eng |
Number of pages: | 10 |
Belongs to series: | Postharvest Biology and Technology |
ISSN: | 0925-5214 |
DOI: | https://doi.org/10.1016/j.postharvbio.2019.02.010 |
URI: | http://hdl.handle.net/10138/327098 |
Abstract: | Diseases develop during the storage of onions. To minimize losses, new methods are needed to identify diseased bulbs early in storage. Volatile organic compounds (VOCs), the respiration rate, weight loss, and the dry matter content were investigated for 1-7 weeks post inoculation of bulbs with water (control) and two strains (Fox006 or Fox260) of Fusarium oxysporum f. sp. cepae. Photos, multispectral image analysis, and real-time polymerase chain reaction (PCR) showed no infection in the control onions, weak pathogenic infection in Fox006-onions, and strong pathogenic infection in Fox260-onions at week 7 post inoculation. Infected bulbs exhibited increased respiration rate, increased VOC emission rate, and increased weight loss. The control and Fox006-onions did not respond to inoculation and had similar reaction pattern. Forty-three different VOCs were measured, of which 17 compounds had sulfur in their chemical structure. 1-Propanethiol, methyl propyl sulfide, and styrene were emitted in high concentrations and were positively correlated with the extent of infection (r = 0.82 - 0.89). Therefore, these compounds were the most promising volatile markers of Fusarium basal rot infection. For the first time, we show that the extent of fungal infection determined by real-time PCR in onion bulbs is related with VOC emission. |
Subject: |
Allium cepa L.
Food spoilage SPME Image analysis Real-time PCR Respiration rate DISCRIMINANT-ANALYSIS PROLIFERATUM QUALITY 1183 Plant biology, microbiology, virology 416 Food Science |
Peer reviewed: | Yes |
Rights: | cc_by_nc_nd |
Usage restriction: | openAccess |
Self-archived version: | acceptedVersion |
Total number of downloads: Loading...
Files | Size | Format | View |
---|---|---|---|
Aimei_Manuscrip ... ec_last_author_version.pdf | 888.3Kb |
View/ |