Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes

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http://hdl.handle.net/10138/327137

Lähdeviite

Prabhakar , N , Belevich , I , Peurla , M , Heiligenstein , X , Chang , H-C , Sahlgren , C , Jokitalo , E & Rosenholm , J M 2020 , ' Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes ' , Nanomaterials , vol. 11 , no. 1 , 14 . https://doi.org/10.3390/nano11010014

Julkaisun nimi: Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes
Tekijä: Prabhakar, Neeraj; Belevich, Ilya; Peurla, Markus; Heiligenstein, Xavier; Chang, Huan-Cheng; Sahlgren, Cecilia; Jokitalo, Eija; Rosenholm, Jessica M.
Muu tekijä: University of Helsinki, Institute of Biotechnology
University of Helsinki, Electron Microscopy
Päiväys: 2020-12-23
Kieli: eng
Sivumäärä: 10
Kuuluu julkaisusarjaan: Nanomaterials
ISSN: 2079-4991
URI: http://hdl.handle.net/10138/327137
Tiivistelmä: Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.
Avainsanat: correlative microscopy
3D CLEM
volume imaging
1182 Biochemistry, cell and molecular biology
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