Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes

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dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Electron Microscopy en
dc.contributor.author Prabhakar, Neeraj
dc.contributor.author Belevich, Ilya
dc.contributor.author Peurla, Markus
dc.contributor.author Heiligenstein, Xavier
dc.contributor.author Chang, Huan-Cheng
dc.contributor.author Sahlgren, Cecilia
dc.contributor.author Jokitalo, Eija
dc.contributor.author Rosenholm, Jessica M.
dc.date.accessioned 2021-02-26T13:40:01Z
dc.date.available 2021-02-26T13:40:01Z
dc.date.issued 2020-12-23
dc.identifier.citation Prabhakar , N , Belevich , I , Peurla , M , Heiligenstein , X , Chang , H-C , Sahlgren , C , Jokitalo , E & Rosenholm , J M 2020 , ' Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes ' , Nanomaterials , vol. 11 , no. 1 , 14 . https://doi.org/10.3390/nano11010014 en
dc.identifier.issn 2079-4991
dc.identifier.other PURE: 160579056
dc.identifier.other PURE UUID: 945021cd-29e9-4699-ac5a-81f57b0efa94
dc.identifier.other WOS: 000610635400001
dc.identifier.other ORCID: /0000-0003-2190-4909/work/89579678
dc.identifier.other ORCID: /0000-0002-4159-6934/work/89583546
dc.identifier.uri http://hdl.handle.net/10138/327137
dc.description.abstract Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Nanomaterials
dc.rights en
dc.subject correlative microscopy en
dc.subject 3D CLEM en
dc.subject volume imaging en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.title Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.3390/nano11010014
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl
dc.contributor.pbl

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