An ethnobotanical survey and antifungal activity of Piper guineense used for the treatment of fungal infections in West-African traditional medicine

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dc.contributor.author Mgbeahuruike, Eunice Ego
dc.contributor.author Holm, Yvonne
dc.contributor.author Vuorela, Heikki
dc.contributor.author Amandikwa, Chinyere
dc.contributor.author Fyhrquist, Pia
dc.date.accessioned 2021-03-03T09:50:01Z
dc.date.available 2021-03-03T09:50:01Z
dc.date.issued 2019-01-30
dc.identifier.citation Mgbeahuruike , E E , Holm , Y , Vuorela , H , Amandikwa , C & Fyhrquist , P 2019 , ' An ethnobotanical survey and antifungal activity of Piper guineense used for the treatment of fungal infections in West-African traditional medicine ' , Journal of Ethnopharmacology , vol. 229 , pp. 157-166 . https://doi.org/10.1016/j.jep.2018.10.005
dc.identifier.other PURE: 121232178
dc.identifier.other PURE UUID: 74dfbfe4-dd83-40ee-886a-6a4b21902d32
dc.identifier.other WOS: 000452343400016
dc.identifier.other Scopus: 85055164046
dc.identifier.uri http://hdl.handle.net/10138/327406
dc.description.abstract Ethnobotanical relevance: Piper guineense occurs commonly in West Africa where it is used for fungal infections instead of the costly and not always accessible conventional antifungals. Fungal, yeast-based diseases are common in West-Africa especially among those living with HIV/AIDS, and thus this study was performed in Imo state, South-Eastern Nigeria, where P. guineense is predominantly used for the treatment of fungal diseases, such as skin rashes, oral thrush and vaginosis. Aim of study: The scarce number of previous studies on the documentation of the traditional uses of P. guineense extracts for the treatment of fungal infections in Nigeria prompted this survey. The investigation focused on how traditional healers recognize and diagnose fungal infections, how P. guineense is collected, on the various parts used for the treatments, methods of preparations, administrations and treatments. In addition, an in vitro anti fungal screening of P. guineense fruit and leaf extracts of various polarities, and piperine and piperlongumine, representing the main constituents in these extracts, were performed. Methods: A house to house ethnobotanical survey was conducted using questionnaires. Twenty traditional medical practitioners (TMP) and herb sellers from ten villages were interviewed. Four human pathogenic strains of yeast and Cryptococcus neoformans, a yeast-like basidiomycete causing meningitis in immunocompromised individuals, were used for the antifungal screening. Results: The traditional medical practitioners (TMP) and herb sellers explained that the leaves and fruits are the most commonly used plant parts for the treatments. The oral intake of the extracts in locally produced bamboo alcohol (Kai-kai) is the most common method of administration. In accordance with these recorded traditional uses, we found that extracts of P. guineense were growth inhibitory against the fungal strains with MIC values ranging from 39 to 2500 g/mL. The lowest MIC value of 39 g/mL was recorded for a methanol fruit extract against Candida albicans, C. glabrata and C. tropicalis. In addition, ethanol and hexane fruit extracts were effective against the growth of C. albicans and C. glabrata, respectively, with a MIC of 78 g/mL. Piperlongumine and piperine were active against C. albicans with MIC values of 39 and 78 g/mL respectively. Conclusion: P. guineense fruit and leaf extracts, as well as their piperamide alkaloid constituents piperine and piperlongumine, have interesting antifungal properties and could have potential as new antifungal scaffolds. Our results warrant further in-depth investigations to isolate and characterize piperamide alkaloids and other compounds responsible for the antifungal activity in the extracts. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Journal of Ethnopharmacology
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Piper guineense
dc.subject Antifungal uses
dc.subject Ethnobotanical survey
dc.subject Antifungal screening
dc.subject Piperine
dc.subject Piperlongumine
dc.subject CANDIDA-ALBICANS
dc.subject PLANTS
dc.subject RESISTANCE
dc.subject PATHOGENS
dc.subject EXTRACTS
dc.subject MALARIA
dc.subject REGION
dc.subject 1183 Plant biology, microbiology, virology
dc.subject 317 Pharmacy
dc.title An ethnobotanical survey and antifungal activity of Piper guineense used for the treatment of fungal infections in West-African traditional medicine en
dc.type Article
dc.contributor.organization Pharmaceutical Design and Discovery group
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Drug Research Program
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.jep.2018.10.005
dc.relation.issn 0378-8741
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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