Next generation organoids for biomedical research and applications

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dc.contributor.author Lou, Yan-Ru
dc.contributor.author Leung, Alan W.
dc.date.accessioned 2021-03-03T10:02:01Z
dc.date.available 2021-03-03T10:02:01Z
dc.date.issued 2018
dc.identifier.citation Lou , Y-R & Leung , A W 2018 , ' Next generation organoids for biomedical research and applications ' , Biotechnology Advances , vol. 36 , no. 1 , pp. 132-149 . https://doi.org/10.1016/j.biotechadv.2017.10.005
dc.identifier.other PURE: 99300389
dc.identifier.other PURE UUID: 954e714d-e4d1-4878-95ed-8e395884f163
dc.identifier.other WOS: 000424182700009
dc.identifier.other Scopus: 85032195013
dc.identifier.other ORCID: /0000-0001-7717-6010/work/42135092
dc.identifier.uri http://hdl.handle.net/10138/327408
dc.description Corrigendum: Biotechnology Advances, Volume 37, Issue 3, May–June 2019, Page 505 DOI: 10.1016/j.biotechadv.2019.03.001 WOS: 000464301900010
dc.description.abstract Organoids are in vitro cultures of miniature fetal or adult organ-like structures. Their potentials for use in tissue and organ replacement, disease modeling, toxicology studies, and drug discovery are tremendous. Currently, major challenges facing human organoid technology include (i) improving the range of cellular heterogeneity for a particular organoid system, (ii) mimicking the native micro- and matrix-environment encountered by cells within organoids, and (iii) developing robust protocols for the in vitro maturation of organoids that remain mostly fetal-like in cultures. To tackle these challenges, we advocate the principle of reverse engineering that replicates the inner workings of in vivo systems with the goal of achieving functionality and maturation of the resulting organoid structures with the input of minimal intrinsic (cellular) and environmental (matrix and niche) constituents. Here, we present an overview of organoid technology development in several systems that employ cell materials derived from fetal and adult tissues and pluripotent stem cell cultures. We focus on key studies that exploit the self-organizing property of embryonic progenitors and the role of designer matrices and cell-free scaffolds in assisting organoid formation. We further explore the relationship between adult stem cells, niche factors, and other current developments that aim to enhance robust organoid maturation. From these works, we propose a standardized pipeline for the development of future protocols that would help generate more physiologically relevant human organoids for various biomedical applications. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof Biotechnology Advances
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Organoids
dc.subject Human
dc.subject Reverse engineering
dc.subject Self-organization
dc.subject Designer matrix
dc.subject Niche factors
dc.subject Pluripotent stem cells
dc.subject Drug screening
dc.subject Disease modeling
dc.subject Transplantation
dc.subject PLURIPOTENT STEM-CELLS
dc.subject IN-VITRO EXPANSION
dc.subject DISSOCIATED EMBRYONIC CHICK
dc.subject HUMAN CEREBRAL ORGANOIDS
dc.subject NEURAL CREST INDUCTION
dc.subject ADVERSE DRUG-REACTIONS
dc.subject FUNCTIONAL HUMAN LIVER
dc.subject HEPATOCYTE-LIKE CELLS
dc.subject EX-VIVO EXPANSION
dc.subject EXTRACELLULAR-MATRIX
dc.subject 317 Pharmacy
dc.title Next generation organoids for biomedical research and applications en
dc.type Review Article
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Drug Research Program
dc.contributor.organization Tissue engineering for drug research
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.biotechadv.2017.10.005
dc.relation.issn 0734-9750
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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