Isolation of dental stem cell-enriched populations from continuously growing mouse incisors

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http://hdl.handle.net/10138/327589

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Balic , A 2019 , Isolation of dental stem cell-enriched populations from continuously growing mouse incisors . in P Papagerakis (ed.) , Odontogenesis : Methods and Protocols . Methods in Molecular Biology , vol. 1922 , Humana press , pp. 29-37 . https://doi.org/10.1007/978-1-4939-9012-2_4

Title: Isolation of dental stem cell-enriched populations from continuously growing mouse incisors
Author: Balic, A.
Other contributor: University of Helsinki, Institute of Biotechnology
Papagerakis, Petros
Publisher: Humana press
Date: 2019
Language: eng
Number of pages: 9
Belongs to series: Odontogenesis Methods and Protocols
Belongs to series: Methods in Molecular Biology
ISBN: 978-1-4939-9011-5
978-1-4939-9012-2
DOI: https://doi.org/10.1007/978-1-4939-9012-2_4
URI: http://hdl.handle.net/10138/327589
Abstract: Continuous growth of the rodent incisor is enabled by epithelial and mesenchymal stem cells (ESCs and MSCs) which unceasingly replenish enamel and dentin, respectively, that wear by persistent animal gnawing. Lineage tracing studies have provided evidence that ESCs contribute to all epithelial lineages of the tooth in vivo. Meanwhile, in the mouse incisor, MSCs continuously contribute to odontoblast lineage and tooth growth. However, in vitro manipulation of ESCs has shown little progress, mainly due to lack of appropriate protocol to successfully isolate, culture, expand, and differentiate ESCs in vitro without using the co-culture system. In this chapter we describe the isolation of the Sox2-GFP+ cell population that is highly enriched in ESCs. Isolated cells can be used for various types of analyses, including in vitro culture, single cell-related analyses, etc. Furthermore, we describe ways to obtain populations enriched in the incisor MSCs using FACS sorting of antibody-labeled cells. Easily accessible FACS sorting enables easy and relatively fast isolation of the cells labeled by the fluorescent protein. © Springer Science+Business Media, LLC, part of Springer Nature 2019.
Subject: Dental tissues
Mouse incisors
Stem cell-enriched population
Tooth regeneration
antibody
Sox2 protein, mouse
transcription factor Sox
animal tissue
cell isolation
cell population
controlled study
dissection
fluorescence activated cell sorting
incisor
mandible
mouse
nonhuman
stem cell
third molar
tooth development
tooth pulp
animal
cell separation
cytology
mesenchymal stem cell
procedures
Animals
Cell Separation
Incisor
Mesenchymal Stem Cells
Mice
SOXB1 Transcription Factors
Stem Cells
313 Dentistry
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