Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species

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Vuorinen , K , Zamora , O , Vaahtera , L , Overmyer , K & Brosché , M 2021 , ' Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species ' , Molecular Plant - Microbe Interactions , vol. 34 , no. 1 , pp. 75-87 . https://doi.org/10.1094/MPMI-07-20-0202-R

Title: Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species
Author: Vuorinen, Katariina; Zamora, Olena; Vaahtera, Lauri; Overmyer, Kirk; Brosché, Mikael
Contributor: University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, University of Tartu
University of Helsinki, Norwegian University of Science and Technology
University of Helsinki, Biosciences
University of Helsinki, Viikki Plant Science Centre (ViPS)
Date: 2021-01
Language: eng
Number of pages: 13
Belongs to series: Molecular Plant - Microbe Interactions
ISSN: 0894-0282
URI: http://hdl.handle.net/10138/327613
Abstract: Plants require interaction between signaling pathways to differentiate and integrate stress responses and deploy appropriate defenses. The hormones ethylene, salicylic acid (SA), and jasmonic acid (JA) are important regulators of plant defenses. Numerous interactions between these signaling pathways are the cornerstone of robust plant immunity. Additionally, during the early response to pathogens, reactive oxygen species (ROS) act as signaling molecules. Here, we examined the extent of signal interaction in the early stages of Botrytis cinerea infection. To enable a comparison between B. cinerea infection with ROS signaling, we subjected plants to ozone treatment, which stimulates an apoplastic ROS burst. We used a collection of single, double, and triple signaling mutants defective in hormone signaling and biosynthesis and subjected them to B. cinerea infection and ozone treatment at different timepoints. We examined lesion size, cell death, and gene expression (both quantitatively and spatially). The two treatments shared many similarities, especially in JA-insensitive mutants, which were sensitive to both treatments. Unexpectedly, a B. cinerea- susceptible JA-insensitive mutant (coil), became tolerant when both SA biosynthesis and signaling was impaired (coil npr1 sid2), demonstrating that JA responses may be under the control of SA. Extensive marker gene analysis indicated JA as the main regulator of both B. cinerea and ozone defenses. In addition, we identified the transcription factor SRI_ as a crucial regulator of PLANT DEFENSIN expression and cell-death regulation, which contributes to resistance to B. cinerea. Overall, our work further defines the context of ROS in plant defense signaling.
Subject: ETHYLENE
GROWTH
JASMONIC ACID
MUTANT
PLANT DEFENSE
PROTEIN
REPRESSION
RESISTANCE
SALICYLIC-ACID
TRANSCRIPTION FACTOR
cell death
defense signaling pathways
ethylene
fungus-plant interactions
jasmonic acid
salicylic acid
1182 Biochemistry, cell and molecular biology
11831 Plant biology
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