Vuorinen , K , Zamora , O , Vaahtera , L , Overmyer , K & Brosché , M 2021 , ' Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species ' , Molecular Plant - Microbe Interactions , vol. 34 , no. 1 , pp. 75-87 . https://doi.org/10.1094/MPMI-07-20-0202-R
Title: | Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species |
Author: | Vuorinen, Katariina; Zamora, Olena; Vaahtera, Lauri; Overmyer, Kirk; Brosché, Mikael |
Contributor organization: | Organismal and Evolutionary Biology Research Programme Faculty of Biological and Environmental Sciences Viikki Plant Science Centre (ViPS) Biosciences Plant-Fungal Interactions Group Plant stress and natural variation Plant Biology |
Date: | 2021-01 |
Language: | eng |
Number of pages: | 13 |
Belongs to series: | Molecular Plant - Microbe Interactions |
ISSN: | 0894-0282 |
DOI: | https://doi.org/10.1094/MPMI-07-20-0202-R |
URI: | http://hdl.handle.net/10138/327613 |
Abstract: | Plants require interaction between signaling pathways to differentiate and integrate stress responses and deploy appropriate defenses. The hormones ethylene, salicylic acid (SA), and jasmonic acid (JA) are important regulators of plant defenses. Numerous interactions between these signaling pathways are the cornerstone of robust plant immunity. Additionally, during the early response to pathogens, reactive oxygen species (ROS) act as signaling molecules. Here, we examined the extent of signal interaction in the early stages of Botrytis cinerea infection. To enable a comparison between B. cinerea infection with ROS signaling, we subjected plants to ozone treatment, which stimulates an apoplastic ROS burst. We used a collection of single, double, and triple signaling mutants defective in hormone signaling and biosynthesis and subjected them to B. cinerea infection and ozone treatment at different timepoints. We examined lesion size, cell death, and gene expression (both quantitatively and spatially). The two treatments shared many similarities, especially in JA-insensitive mutants, which were sensitive to both treatments. Unexpectedly, a B. cinerea- susceptible JA-insensitive mutant (coil), became tolerant when both SA biosynthesis and signaling was impaired (coil npr1 sid2), demonstrating that JA responses may be under the control of SA. Extensive marker gene analysis indicated JA as the main regulator of both B. cinerea and ozone defenses. In addition, we identified the transcription factor SRI_ as a crucial regulator of PLANT DEFENSIN expression and cell-death regulation, which contributes to resistance to B. cinerea. Overall, our work further defines the context of ROS in plant defense signaling. |
Subject: |
ETHYLENE
GROWTH JASMONIC ACID MUTANT PLANT DEFENSE PROTEIN REPRESSION RESISTANCE SALICYLIC-ACID TRANSCRIPTION FACTOR cell death defense signaling pathways ethylene fungus-plant interactions jasmonic acid salicylic acid 1182 Biochemistry, cell and molecular biology 11831 Plant biology |
Peer reviewed: | Yes |
Rights: | cc_by_nc_nd |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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