Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species

Visa fullständig post



Permalänk

http://hdl.handle.net/10138/327613

Citation

Vuorinen , K , Zamora , O , Vaahtera , L , Overmyer , K & Brosché , M 2021 , ' Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species ' , Molecular Plant - Microbe Interactions , vol. 34 , no. 1 , pp. 75-87 . https://doi.org/10.1094/MPMI-07-20-0202-R

Titel: Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species
Författare: Vuorinen, Katariina; Zamora, Olena; Vaahtera, Lauri; Overmyer, Kirk; Brosché, Mikael
Upphovmannens organisation: Organismal and Evolutionary Biology Research Programme
Faculty of Biological and Environmental Sciences
Viikki Plant Science Centre (ViPS)
Biosciences
Plant-Fungal Interactions Group
Plant stress and natural variation
Plant Biology
Datum: 2021-01
Språk: eng
Sidantal: 13
Tillhör serie: Molecular Plant - Microbe Interactions
ISSN: 0894-0282
DOI: https://doi.org/10.1094/MPMI-07-20-0202-R
Permanenta länken (URI): http://hdl.handle.net/10138/327613
Abstrakt: Plants require interaction between signaling pathways to differentiate and integrate stress responses and deploy appropriate defenses. The hormones ethylene, salicylic acid (SA), and jasmonic acid (JA) are important regulators of plant defenses. Numerous interactions between these signaling pathways are the cornerstone of robust plant immunity. Additionally, during the early response to pathogens, reactive oxygen species (ROS) act as signaling molecules. Here, we examined the extent of signal interaction in the early stages of Botrytis cinerea infection. To enable a comparison between B. cinerea infection with ROS signaling, we subjected plants to ozone treatment, which stimulates an apoplastic ROS burst. We used a collection of single, double, and triple signaling mutants defective in hormone signaling and biosynthesis and subjected them to B. cinerea infection and ozone treatment at different timepoints. We examined lesion size, cell death, and gene expression (both quantitatively and spatially). The two treatments shared many similarities, especially in JA-insensitive mutants, which were sensitive to both treatments. Unexpectedly, a B. cinerea- susceptible JA-insensitive mutant (coil), became tolerant when both SA biosynthesis and signaling was impaired (coil npr1 sid2), demonstrating that JA responses may be under the control of SA. Extensive marker gene analysis indicated JA as the main regulator of both B. cinerea and ozone defenses. In addition, we identified the transcription factor SRI_ as a crucial regulator of PLANT DEFENSIN expression and cell-death regulation, which contributes to resistance to B. cinerea. Overall, our work further defines the context of ROS in plant defense signaling.
Subject: ETHYLENE
GROWTH
JASMONIC ACID
MUTANT
PLANT DEFENSE
PROTEIN
REPRESSION
RESISTANCE
SALICYLIC-ACID
TRANSCRIPTION FACTOR
cell death
defense signaling pathways
ethylene
fungus-plant interactions
jasmonic acid
salicylic acid
1182 Biochemistry, cell and molecular biology
11831 Plant biology
Referentgranskad: Ja
Licens: cc_by_nc_nd
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


Filer under denna titel

Totalt antal nerladdningar: Laddar...

Filer Storlek Format Granska
mpmi_07_20_0202_r.pdf 2.134Mb PDF Granska/Öppna

Detta dokument registreras i samling:

Visa fullständig post