Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines

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http://hdl.handle.net/10138/327709

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de Aquino , I G , Bastos , D C , Cuadra-Zelaya , F J M , Teixeira , I F , Salo , T , Coletta , R D & Graner , E 2020 , ' Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines ' , Archives of Oral Biology , vol. 113 , 104707 . https://doi.org/10.1016/j.archoralbio.2020.104707

Title: Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines
Author: de Aquino, Iara Gonçalves; Bastos, Débora Campanella; Cuadra-Zelaya, Florence Juana Maria; Teixeira, Isadora Ferrari; Salo, Tuula; Coletta, Ricardo Della; Graner, Edgard
Contributor: University of Helsinki, HUSLAB
Date: 2020-05
Language: eng
Number of pages: 7
Belongs to series: Archives of Oral Biology
ISSN: 0003-9969
URI: http://hdl.handle.net/10138/327709
Abstract: Objective Fatty acid synthase (FASN) is overexpressed in several human cancers, including oral squamous cell carcinoma (OSCC). TVB-3166 is a recently described FASN inhibitor with antitumor effects and potential clinical relevance. The objective of this study was to evaluate the effects of TVB-3166 on OSCC cell lines. Materials and methods The OSCC cell line SCC-9 modified to express ZsGreen (ZsG) (SCC-9 ZsG) and its in vivo selected metastatic derivative LN-1A were used to evaluate anticancer properties of TVB-3166. Cell viability was determined using MTT assays and proliferation determined by cell counting in a Neubauer chamber. Cell death and cell cycle progression were analyzed by Annexin V-PE/7-ADD-PerCP labeling and PI staining, respectively. Cell migration was assayed by scratch assays and cell adhesion using myogel. Production of FASN, p-AKT, CPT1-α, and epithelial-mesenchymal transition (EMT) markers were examined by Western blotting. Results TVB-3166 significantly reduced cell viability and proliferation, promoted cell cycle arrest and apoptosis, and increased adhesion to myogel in both OSCC cell lines. Finally, the drug reduced SCC-9 ZsG migration. Conclusion Our results demonstrated that TVB-3166 has anticancer effects on both SCC-9 ZsG and its metastatic version LN-1A, which are worthy of investigation in preclinical models for OSCC.
Subject: 3122 Cancers
TVB-3166
fatty acid synthase
oral squamous cell carcinoma
cell lines
APOPTOSIS
Oral squamous cell carcinoma
BREAST-CANCER CELLS
IN-VITRO
DISRUPTION
LYMPH-NODE METASTASIS
METABOLISM
FASN
Cell lines
THERAPEUTIC TARGET
GROWTH
Fatty acid synthase
EXPRESSION
313 Dentistry
3126 Surgery, anesthesiology, intensive care, radiology
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