Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I : Wet lab procedure

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ESCV Network Next-Generation Seque, Xavier Lopez-Labrador, F, Brown, J R, Fischer, N, Auvinen, E & de Vries, J J C 2021, ' Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I : Wet lab procedure ', Journal of Clinical Virology, vol. 134, 104691 . https://doi.org/10.1016/j.jcv.2020.104691

Title: Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I : Wet lab procedure
Author: ESCV Network Next-Generation Seque; Xavier Lopez-Labrador, F.; Brown, Julianne R.; Fischer, Nicole; Auvinen, Eeva; de Vries, Jutte J. C.
Contributor organization: Medicum
Eeva Auvinen / Principal Investigator
Biosciences
HUSLAB
Department of Virology
University of Helsinki
Helsinki University Hospital Area
Date: 2021-01
Language: eng
Number of pages: 8
Belongs to series: Journal of Clinical Virology
ISSN: 1386-6532
DOI: https://doi.org/10.1016/j.jcv.2020.104691
URI: http://hdl.handle.net/10138/327884
Abstract: Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
Subject: Viral metagenomics
High-throughput sequencing
Next-generation sequencing
Wet lab
Recommendations
INFLUENZA-A VIRUS
EFFICIENT TRANSLATION
PATHOGEN DETECTION
MESSENGER-RNA
DNA
VALIDATION
DIAGNOSIS
DEPLETION
3111 Biomedicine
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: publishedVersion


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