Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments

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Yan , Y , Stoddard , F L , Neugart , S , Sadras , V O , Lindfors , A , Morales , L O & Aphalo , P J 2019 , ' Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments ' , Photochemical & Photobiological Sciences , vol. 18 , no. 2 , pp. 434-447 . https://doi.org/10.1039/c8pp00567b

Title: Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments
Author: Yan, Yan; Stoddard, Frederick L.; Neugart, Susanne; Sadras, Victor O.; Lindfors, Anders; Morales, Luis Orlando; Aphalo, Pedro J.
Other contributor: University of Helsinki, Plant Biology
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Organismal and Evolutionary Biology Research Programme









Date: 2019-02-01
Language: eng
Number of pages: 14
Belongs to series: Photochemical & Photobiological Sciences
ISSN: 1474-905X
DOI: https://doi.org/10.1039/c8pp00567b
URI: http://hdl.handle.net/10138/327987
Abstract: Blue light and UV radiation shape a plant's morphology and development, but accession-dependent responses under natural conditions are unclear. Here we tested the hypothesis that two faba bean (Vicia faba L.) accessions adapted to different latitudes and altitudes vary in their responses to solar blue and UV light. We measured growth, physiological traits, phenolic profiles and expression of associated genes in a factorial experiment combining two accessions (Aurora, a Swedish cultivar adapted to high latitude and low altitude; ILB938, from the Andean region of Colombia and Ecuador, adapted to low latitude and high altitude) and four filter treatments created with plastic sheets: 1. transparent as control; 2. attenuated short UV (290-350 nm); 3. attenuated UV (290-400 nm); 4. attenuated blue and UV light. In both accessions, the exclusion of blue and UV light increased plant height and leaf area, and decreased transcript abundance of ELONGATED HYPOCOTYL 5 (HY5) and TYROSINE AMINOTRANSFERASE 3 (TAT3). Blue light and short UV induced the accumulation of epidermal and whole-leaf flavonoids, mainly quercetins, and the responses in the two accessions were through different glycosides. Filter treatments did not affect kaempferol concentration, but there were more tri-glycosides in Aurora and di-glycosides in ILB938. Furthermore, fewer quercetin glycosides were identified in ILB938. The transcript abundance was consistently higher in Aurora than in ILB938 for all seven investigated genes: HY5, TAT3, CHALCONE SYNTHASE (CHS), CHALCONE ISOMERASE (CHI), DON-GLUCOSYLTRANSFERASE 1 (DOGT1), ABA INSENSITIVE 2 (ABI2), AUXIN-INDUCIBLE 2-27 (IAA5). The two largest differences in transcript abundance between the two accessions across treatments were 132-fold in CHS and 30-fold in DOGT1 which may explain the accession-dependent glycosylation patterns. Our findings suggest that agronomic selection for adaptation to high altitude may favour phenotypes with particular adaptations to the light environment, including solar UV and blue light.
Subject: LIGHT SIGNAL-TRANSDUCTION
ULTRAVIOLET-B RADIATION
PLANT-GROWTH
ARABIDOPSIS
ROLES
PERCEPTION
IDENTIFICATION
ACCUMULATION
CRYPTOCHROME
GLYCOSIDES
1182 Biochemistry, cell and molecular biology
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