Rotational Diffusion of Membrane Proteins in Crowded Membranes

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Javanainen , M , Ollila , O H S & Martinez-Seara , H 2020 , ' Rotational Diffusion of Membrane Proteins in Crowded Membranes ' , Journal of Physical Chemistry B , vol. 124 , no. 15 , pp. 2994-3001 .

Title: Rotational Diffusion of Membrane Proteins in Crowded Membranes
Author: Javanainen, Matti; Ollila, O. H. Samuli; Martinez-Seara, Hector
Contributor organization: Biophysical chemistry
Institute of Biotechnology
Date: 2020-04-16
Language: eng
Number of pages: 8
Belongs to series: Journal of Physical Chemistry B
ISSN: 1520-6106
Abstract: Membrane proteins travel along cellular membranes and reorient themselves to form functional oligomers and proteinlipid complexes. Following the Saffman-Delbruck model, protein-radius sets the rate of this diffusive motion. However, it is unclear how this model, derived for ideal and dilute membranes, performs under crowded conditions of cellular membranes. Here, we study the rotational motion of membrane proteins using molecular dynamics simulations of coarse-grained membranes and 2-dimensional Lennard-Jones fluids with varying levels of crowding. We find that the Saffman-Delbruck model captures the size-dependency of rotational diffusion under dilute conditions where protein-protein interactions are negligible, whereas stronger scaling laws arise under crowding. Together with our recent work on lateral diffusion, our results reshape the description of protein dynamics in native membrane environments: The translational and rotational motions of proteins with small transmembrane domains are rapid, whereas larger proteins or protein complexes display substantially slower dynamics.
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion

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