Systematic in vitro biocompatibility studies of multimodal cellulose nanocrystal and lignin nanoparticles

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Imlimthan , S , Correia , A , Figueiredo , P I , Lintinen , K , Balasubramanian , V , Airaksinen , A , Kostiainen , M A , Almeida Santos , H & Sarparanta , M P 2020 , ' Systematic in vitro biocompatibility studies of multimodal cellulose nanocrystal and lignin nanoparticles ' , Journal of Biomedical Materials Research. Part A , vol. 108 , no. 3 , pp. 770-783 .

Title: Systematic in vitro biocompatibility studies of multimodal cellulose nanocrystal and lignin nanoparticles
Author: Imlimthan, Surachet; Correia, Alexandra; Figueiredo, Patricia Isabel; Lintinen, Kalle; Balasubramanian, Vimalkumar; Airaksinen, Anu; Kostiainen, Mauri A.; Almeida Santos, Helder; Sarparanta, Mirkka Päivikki
Contributor organization: Department of Chemistry
Tracers in Molecular Imaging (TRIM)
Nanomedicines and Biomedical Engineering
Division of Pharmaceutical Chemistry and Technology
Drug Research Program
Helsinki In Vivo Animal Imaging Platform (HAIP)
Helsinki One Health (HOH)
Helsinki Institute of Life Science HiLIFE
Date: 2020-03
Language: eng
Number of pages: 14
Belongs to series: Journal of Biomedical Materials Research. Part A
ISSN: 1549-3296
Abstract: Natural biopolymer nanoparticles (NPs), including nanocrystalline cellulose (CNC) and lignin, have shown potential as scaffolds for targeted drug delivery systems due to their wide availability, cost‐efficient preparation, and anticipated biocompatibility. Since both CNC and lignin can potentially cause complications in cell viability assays due to their ability to scatter the emitted light and absorb the assay reagents, we investigated the response of bioluminescent (CellTiter‐Glo®), colorimetric (MTT® and AlamarBlue®) and fluorometric (LIVE/DEAD®) assays for the determination of the biocompatibility of the multimodal CNC and lignin constructs in murine RAW 264.7 macrophages and 4T1 breast adenocarcinoma cell lines. Here, we have developed multimodal CNC and lignin NPs harboring the radiometal chelator DOTA (1,4,7,10‐tetraazacyclododecane‐1,4,7,10‐tetraacetic acid) and the fluorescent dye Cyanine 5 for the investigation of nanomaterial biodistribution in vivo with nuclear and optical imaging, which were then used as the model CNC and lignin nanosystems in the cell viability assay comparison. CellTiter‐Glo® based on the detection of ATP‐dependent luminescence in viable cells revealed to be the best assay for both nanoconstructs for its robust linear response to increasing NP concentration and lack of interference from either of the NP types. Both multimodal CNC and lignin NPs displayed low cytotoxicity and favorable interactions with the cell lines, suggesting that they are good candidates for nanosystem development for targeted drug delivery in breast cancer and for theranostic applications. Our results provide useful guidance for cell viability assay compatibility for CNC and lignin NPs and facilitate the future translation of the materials for in vivo applications.
Subject: AGENT
cell viability
cellulose nanocrystal
lignin nanoparticle
multimodal imaging probe
radiopharmaceutical chemistry
drug delivery system
nanocrystalline cellulose
116 Chemical sciences
317 Pharmacy
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion
Funder: Valtion perusrahoitus/hankkeet
Grant number:

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