Gingival tissue human beta-defensin levels in relation to infection and inflammation.

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http://hdl.handle.net/10138/328536

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Özdemir , M , Caglayan , F , Bigger , F , Pussinen , P , Könönen , E , Yamalik , N , Gursoy , M , Fteita , D , Nazmi , K , Güncü , G N , Pietiäinen , M , Tolvanen , M & Gürsoy , U K 2020 , ' Gingival tissue human beta-defensin levels in relation to infection and inflammation. ' , Journal of Clinical Periodontology , vol. 47 , no. 3 , pp. 309-318 . https://doi.org/10.1111/jcpe.13227

Title: Gingival tissue human beta-defensin levels in relation to infection and inflammation.
Author: Özdemir, Meltem; Caglayan, Feriha; Bigger, Floris; Pussinen, Pirkko; Könönen, Eija; Yamalik, Nermin; Gursoy, Mervi; Fteita, Dareen; Nazmi, Kamran; Güncü, Güliz N.; Pietiäinen, Milla; Tolvanen, Mimmi; Gürsoy, Ulvi Kahraman
Contributor: University of Helsinki, HUS Head and Neck Center
University of Helsinki, Department of Oral and Maxillofacial Diseases
Date: 2020-03
Language: eng
Number of pages: 10
Belongs to series: Journal of Clinical Periodontology
ISSN: 0303-6979
URI: http://hdl.handle.net/10138/328536
Abstract: Aim To profile gingival tissue levels of human beta-defensin (hBD)-2 and hBD-3 in relation to gingival inflammation, Th17-related cytokine concentrations, Porphyromonas gingivalis counts, and gingipain and total protease activities. Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with marginal, mild, or moderate to severe inflammation. hBD levels were determined by immunodetection, P. gingivalis counts with real-time polymerase chain reaction, protease activities with fluorogenic substrates, and cytokine concentrations with Luminex technique. Data were statistically analysed using Kruskal-Wallis and Mann-Whitney U tests and Spearman correlation coefficients. Results Subgingival plaque counts of P. gingivalis (p = .001) and gingipain activity (p <.001), as well as interleukin (IL)-1 beta (p = .012), IL-10 (p = .024), IL-17A (p = .002), IL-17F (p = .006), and IL-23 (p = .036) concentrations were elevated in severely inflamed sites, whereas no change was observed in hBD-2 and hBD-3 levels. Negative correlations were found between protease activity and hBD-2 (p = .033) and hBD-3(p = .003) levels. Conclusions Shift in gingival inflammation from marginal to mild stage is related to elevations in subgingival plaque P. gingivalis counts and gingipain activity, but not to tissue hBD levels. Negative correlations between hBDs and total protease activity suggest the degradation of these antimicrobial peptides in progressed inflammation.
Subject: 313 Dentistry
ANTIMICROBIAL PEPTIDES
gingipain
hBD-2
hBD-3
INFLAMMATORY CYTOKINES
PERIODONTITIS
Porphyromonas gingivalis
antimicrobial peptides
gingipain
hBD-2
hBD-3
inflammatory cytokines
periodontitis
Porphyromonas gingivalis
PORPHYROMONAS-GINGIVALIS
HUMAN BETA-DEFENSIN-2
ANTIMICROBIAL PEPTIDES
PERIODONTAL PATHOGENS
EXPRESSION
DISEASE
CULTURE
INTERLEUKIN-8
BIOFILM
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