Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

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Chroumpi , T , Peng , M , Markillie , L M , Mitchell , H D , Nicora , C D , Hutchinson , C M , Paurus , V , Tolic , N , Clendinen , C S , Orr , G , Baker , S E , Makela , M R & de Vries , R P 2021 , ' Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates ' , Frontiers in Bioengineering and Biotechnology , vol. 9 , 644216 . https://doi.org/10.3389/fbioe.2021.644216

Title: Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates
Author: Chroumpi, Tania; Peng, Mao; Markillie, Lye Meng; Mitchell, Hugh D.; Nicora, Carrie D.; Hutchinson, Chelsea M.; Paurus, Vanessa; Tolic, Nikola; Clendinen, Chaevien S.; Orr, Galya; Baker, Scott E.; Makela, Miia R.; de Vries, Ronald P.
Contributor: University of Helsinki, Department of Microbiology
Date: 2021-03-08
Language: eng
Number of pages: 14
Belongs to series: Frontiers in Bioengineering and Biotechnology
ISSN: 2296-4185
URI: http://hdl.handle.net/10138/328630
Abstract: The filamentous ascomycete Aspergillus niger has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains, Delta larA Delta xyrA Delta xyrB, Delta ladA Delta xdhA Delta sdhA and Delta xkiA, grown on wheat bran (WB) and sugar beet pulp (SBP), was evaluated. Our results showed that despite the absolute impact of these PCP mutations on pure pentose sugars, they are not as critical for growth of A. niger on more complex biomass substrates, such as WB and SBP. However, significant phenotypic variation was observed between the two biomass substrates, but also between the different PCP mutants. This shows that the high sugar heterogeneity of these substrates in combination with the high complexity and adaptability of the fungal sugar metabolism allow for activation of alternative strategies to support growth.
Subject: lignocellulosic substrates
pentose catabolic pathway
D-galacturonic acid catabolic pathway
L-rhamnose catabolic pathway
wheat bran
sugar beet pulp
CAZymes
Aspergillus niger
220 Industrial biotechnology
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