Miniscope in vivo imaging of neuronal ensembles in the central amygdala in mice

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http://urn.fi/URN:NBN:fi:hulib-202104071859
Title: Miniscope in vivo imaging of neuronal ensembles in the central amygdala in mice
Author: Verle, Maarten
Contributor: University of Helsinki, Faculty of Biological and Environmental Sciences
Publisher: Helsingin yliopisto
Date: 2021
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-202104071859
http://hdl.handle.net/10138/328808
Thesis level: master's thesis
Degree program: Neurotieteen maisteriohjelma
Master's Programme in Neuroscience
Magisterprogrammet i neurovetenskap
Specialisation: Neurotiede
Neuroscience
Neurovetenskap
Abstract: Advancements in both calcium indicators and optical instrumentation have led to new in vivo techniques, such as Miniscopes, capable of recording the spatiotemporal activity of multiple neurons during unrestrained behaviour in rodents. With these microendoscopic techniques, neuronal populations can be stably recorded over multiple sessions. As a result, Miniscopes allow for the investigation of a brain region’s changing activity patterns as a result of disease progression or behaviour. Recently, open source Miniscope initiatives have led to affordable and accessible versions of this technique. In addition, the collaborative open-source community facilitates rapidly evolving modifications, implementations and designs. Notwithstanding the potential and ever-increasing popularity of Miniscopes, the technique is still in its infancy and not widespread. This study consisted of a background review and a pilot study attempting to image neuronal ensembles in the central nucleus of the amygdala (CeA) using the open-source UCLA V3 Miniscope in mice. Despite not being able to successfully record neuronal activity in the CeA, the study has made progress in generating a protocol for Miniscope implementation at the Pharmacology department of Helsinki. Moreover, the study proposes different adjustments that might be implemented in the future. With the continuation of a synergistic collaboration with the Department of Psychology at the university of Jyväskylä, it is likely that both departments will be able to effectively implement the Miniscope technique in the foreseeable future.
Subject: Miniscope
in vivo imaging
GRIN lens
central amygdala
GCaMP


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