T4-like Bacteriophages Isolated from Pig Stools Infect Yersinia pseudotuberculosis and Yersinia pestis Using LPS and OmpF as Receptors

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dc.contributor.author Salem, Mabruka
dc.contributor.author Pajunen, Maria
dc.contributor.author Jun, Jin Woo
dc.contributor.author Skurnik, Mikael
dc.date.accessioned 2021-04-08T14:09:01Z
dc.date.available 2021-04-08T14:09:01Z
dc.date.issued 2021-02
dc.identifier.citation Salem , M , Pajunen , M , Jun , J W & Skurnik , M 2021 , ' T4-like Bacteriophages Isolated from Pig Stools Infect Yersinia pseudotuberculosis and Yersinia pestis Using LPS and OmpF as Receptors ' , Viruses-Basel , vol. 13 , no. 2 , 296 . https://doi.org/10.3390/v13020296
dc.identifier.other PURE: 161908624
dc.identifier.other PURE UUID: ecc8ddc5-aaf2-4158-9c03-f882fd64fddb
dc.identifier.other WOS: 000623302100001
dc.identifier.other ORCID: /0000-0001-8791-9260/work/91927753
dc.identifier.other ORCID: /0000-0001-5484-2228/work/91928561
dc.identifier.uri http://hdl.handle.net/10138/328851
dc.description.abstract The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. The phages exhibited relatively wide host ranges among Yersinia pseudotuberculosis and related species. One-step growth curve experiments revealed that the phages have latent periods of 50-80 min with burst sizes of 44-65 virions per infected cell. The phage genomes consist of circularly permuted dsDNA of 169,060, 167,058, and 167,132 bp in size, respectively, with a G + C content 35.3%. The number of predicted genes range from 267 to 271. The phage genomes are 84-92% identical to each other and ca 85% identical to phage T4. The phage receptors were identified by whole genome sequencing of spontaneous phage-resistant mutants. The phage-resistant strains had mutations in the ompF, galU, hldD, or hldE genes. OmpF is a porin, and the other genes encode lipopolysaccharide (LPS) biosynthetic enzymes. The ompF, galU, and hldE mutants were successfully complemented in trans with respective wild-type genes. The host recognition was assigned to long tail fiber tip protein Gp38, analogous to that of T-even phages such as Salmonella phage S16, specifically to the distal beta-helices connecting loops. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Viruses-Basel
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Y
dc.subject pseudotuberculosis
dc.subject bacteriophage
dc.subject receptor
dc.subject Myoviridae
dc.subject lipopolysaccharide
dc.subject tail fiber
dc.subject Gp38
dc.subject 3111 Biomedicine
dc.subject 11832 Microbiology and virology
dc.title T4-like Bacteriophages Isolated from Pig Stools Infect Yersinia pseudotuberculosis and Yersinia pestis Using LPS and OmpF as Receptors en
dc.type Article
dc.contributor.organization HUMI - Human Microbiome Research
dc.contributor.organization Research Programs Unit
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Medicum
dc.contributor.organization Glycoscience Group
dc.contributor.organization Helsinki One Health (HOH)
dc.contributor.organization Mikael Skurnik / Principal Investigator
dc.contributor.organization HUSLAB
dc.contributor.organization Faculty of Medicine
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki University Hospital Area
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/v13020296
dc.relation.issn 1999-4915
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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