Correlations between microbiota and metabolites after faecal microbiota transfer in irritable bowel syndrome

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Holster , S , Repsilber , D , Geng , D , Hyotylainen , T , Salonen , A , Lindqvist , C M , Rajan , S K , de Vos , W M , Brummer , R J & König , J 2021 , ' Correlations between microbiota and metabolites after faecal microbiota transfer in irritable bowel syndrome ' , Beneficial Microbes , vol. 12 , no. 1 , pp. 17-30 . https://doi.org/10.3920/BM2020.0010

Title: Correlations between microbiota and metabolites after faecal microbiota transfer in irritable bowel syndrome
Author: Holster, S.; Repsilber, D.; Geng, D.; Hyotylainen, T.; Salonen, A.; Lindqvist, C. M.; Rajan, S. K.; de Vos, W. M.; Brummer, R. J.; König, J.
Contributor: University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Department of Bacteriology and Immunology
Date: 2021
Language: eng
Number of pages: 14
Belongs to series: Beneficial Microbes
ISSN: 1876-2883
URI: http://hdl.handle.net/10138/329182
Abstract: Faecal microbiota transfer (FMT) consists of the infusion of donor faecal material into the intestine of a patient with the aim to restore a disturbed gut microbiota. In this study, it was investigated whether FMT has an effect on faecal microbial composition, its functional capacity, faecal metabolite profiles and their interactions in 16 irritable bowel syndrome (IBS) patients. Faecal samples from eight different time points before and until six months after allogenic FMT (faecal material from a healthy donor) as well as autologous FMT (own faecal material) were analysed by 16S RNA gene amplicon sequencing and gas chromatography coupled to mass spectrometry (GS-MS). The results showed that the allogenic FMT resulted in alterations in the microbial composition that were detectable up to six months, whereas after autologous FMT this was not the case. Similar results were found for the functional profiles, which were predicted from the phylogenetic sequencing data. While both allogenic FMT as well as autologous FMT did not have an effect on the faecal metabolites measured in this study, correlations between the microbial composition and the metabolites showed that the microbe-metabolite interactions seemed to be disrupted after allogenic FMT compared to autologous FMT. This shows that FMT can lead to altered interactions between the gut microbiota and its metabolites in IBS patients. Further research should investigate if and how this affects efficacy of FMT treatments.
Subject: microbe-metabolite interactions
colonic microbiota
metabolite profiles
faecal microbiota transplantation
ACTIVE ULCERATIVE-COLITIS
AMINO-ACID-METABOLISM
INTESTINAL MICROBIOTA
INSULIN SENSITIVITY
TRANSPLANTATION
REMISSION
NETWORKS
BACTERIA
PLACEBO
DONOR
11832 Microbiology and virology
3143 Nutrition
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