Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

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Kurek , M , Akesson , E , Yoshihara , M , Oliver , E , Cui , Y , Becker , M , Alves-Lopes , J P , Bjarnason , R , Romerius , P , Sundin , M , Noren Nystrom , U , Langenskiold , C , Vogt , H , Henningsohn , L , Petersen , C , Soder , O , Guo , J , Mitchell , R T , Jahnukainen , K & Stukenborg , J-B 2021 , ' Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation ' , Cells , vol. 10 , no. 2 , 241 . https://doi.org/10.3390/cells10020241

Title: Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
Author: Kurek, Magdalena; Akesson, Elisabet; Yoshihara, Masahito; Oliver, Elizabeth; Cui, Yanhua; Becker, Martin; Alves-Lopes, Joao Pedro; Bjarnason, Ragnar; Romerius, Patrik; Sundin, Mikael; Noren Nystrom, Ulrika; Langenskiold, Cecilia; Vogt, Hartmut; Henningsohn, Lars; Petersen, Cecilia; Soder, Olle; Guo, Jingtao; Mitchell, Rod T.; Jahnukainen, Kirsi; Stukenborg, Jan-Bernd
Contributor organization: Children's Hospital
Clinicum
HUS Children and Adolescents
University of Helsinki
Helsinki University Hospital Area
Date: 2021-02
Language: eng
Number of pages: 18
Belongs to series: Cells
ISSN: 2073-4409
DOI: https://doi.org/10.3390/cells10020241
URI: http://hdl.handle.net/10138/329356
Abstract: Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.
Subject: germ cells
basal membrane
stem cell niche
seminiferous tubules
infertility
late effects
spermatogonia
Sertoli cells
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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