Enhanced lignocellulolytic enzyme activities on hardwood and softwood during interspecific interactions of white- and brown-rot fungi

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Sugano , J , Maina , N , Wallenius , J & Hilden , K 2021 , ' Enhanced lignocellulolytic enzyme activities on hardwood and softwood during interspecific interactions of white- and brown-rot fungi ' , Journal of fungi , vol. 7 , no. 4 , 265 . https://doi.org/10.3390/jof7040265

Title: Enhanced lignocellulolytic enzyme activities on hardwood and softwood during interspecific interactions of white- and brown-rot fungi
Author: Sugano, Junko; Maina, Ndegwa; Wallenius, Janne; Hilden, Kristiina
Contributor organization: Department of Microbiology
Department of Food and Nutrition
Carbohydrate Chemistry and Enzymology
Grain Technology
Fungal Genetics and Biotechnology
Date: 2021-03
Language: eng
Number of pages: 17
Belongs to series: Journal of fungi
ISSN: 2309-608X
DOI: https://doi.org/10.3390/jof7040265
URI: http://hdl.handle.net/10138/329368
Abstract: Wood decomposition is a sophisticated process where various biocatalysts act simultaneously and synergistically on biopolymers to efficiently break down plant cell walls. In nature, this process depends on the activities of the wood-inhabiting fungal communities that co-exist and interact during wood decay. Wood-decaying fungal species have traditionally been classified as white-rot and brown-rot fungi, which differ in their decay mechanism and enzyme repertoire. To mimic the species interaction during wood decomposition, we have cultivated the white-rot fungus, Bjerkandera adusta, and two brown-rot fungi, Gloeophyllum sepiarium and Antrodia sinuosa, in single and co-cultivations on softwood and hardwood. We compared their extracellular hydrolytic carbohydrate-active and oxidative lignin-degrading enzyme activities and production profiles. The interaction of white-rot and brown-rot species showed enhanced (hemi)cellulase activities on birch and spruce-supplemented cultivations. Based on the enzyme activity profiles, the combination of B. adusta and G. sepiarium facilitated birch wood degradation, whereas B. adusta and A. sinuosa is a promising combination for efficient degradation of spruce wood, showing synergy in beta-glucosidase (BGL) and alpha-galactosidase (AGL) activity. Synergistic BGL and AGL activity was also detected on birch during the interaction of brown-rot species. Our findings indicate that fungal interaction on different woody substrates have an impact on both simultaneous and sequential biocatalytic activities.
Subject: 11832 Microbiology and virology
wood decay
lignocellulose
carbohydrate active enzyme
laccase
white rot
brown rot
synergy
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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