HPV infection and bacterial microbiota in the semen from healthy men

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dc.contributor.author Tuominen, Heidi
dc.contributor.author Rautava, Jaana
dc.contributor.author Kero, Katja
dc.contributor.author Syrjänen, Stina
dc.contributor.author Collado, Maria C
dc.contributor.author Rautava, Samuli
dc.date.accessioned 2021-04-25T03:19:26Z
dc.date.available 2021-04-25T03:19:26Z
dc.date.issued 2021-04-21
dc.identifier.citation BMC Infectious Diseases. 2021 Apr 21;21(1):373
dc.identifier.uri http://hdl.handle.net/10138/329378
dc.description.abstract Abstract Background Aberrant microbiota composition has been linked to disease development at numerous anatomical sites. Microbiota changes in reaction to viral infections, such as human papillomavirus (HPV), have been investigated almost exclusively in the female reproductive tract. However, HPV infection may also affect male health by reducing semen quality and fertility. The aim of this study was to investigate whether present HPV DNA is associated with detectable changes in semen bacterial microbiota composition and diversity. Methods This study relied on stored semen samples from 31 fertile healthy men who participated in the Finnish family HPV Study during the years 1998–2001. DNA was extracted from semen with PCR template preparation kit. HPV was genotyped using Luminex-based Multimetrix® assay. Microbiota was analyzed from the V3-V4 region of 16S rDNA gene following sequencing on an Illumina MiSeq platform. All statistical analyses were performed with Calypso software version 8.84. Results HPV DNA was detected in 19.4% (6/31) of the semen samples. HPV status in the semen did not impact the α-diversity estimations, as measured by Chao1 and Shannon indices, nor ß-diversity. Nevertheless, HPV-positive semen samples exhibited differences in the taxonomic composition of the bacterial microbiota including higher abundances of Moraxellaceae (p = 0.028), Streptococcus (p = 0.0058) and Peptostreptococcus (p = 0.012) compared to HPV-negative semen samples. Conclusion HPV infection is associated with altered bacterial microbiota composition in semen, and this might have in impact to male health in general. As of present, it is unclear whether these changes result from HPV infection or whether altered bacterial microbiota increases susceptibility to HPV infection. More research is needed on viral-bacterial interactions in the male reproductive system.
dc.publisher BioMed Central
dc.subject Bacteria
dc.subject HPV
dc.subject Men
dc.subject Microbiome
dc.subject Microbiota
dc.subject Semen
dc.title HPV infection and bacterial microbiota in the semen from healthy men
dc.date.updated 2021-04-25T03:19:26Z
dc.language.rfc3066 en
dc.rights.holder The Author(s)
dc.type.uri http://purl.org/eprint/entityType/ScholarlyWork
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/type/JournalArticle

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