Verification of UriSed 3 PRO automated urine microscope in regional laboratory environment

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Kouri , T , Alagrund , K , Lehtonen , M , Tohmola , N , Pihlajamaa , T , Kouri , V-P , Friman , S , Holma , T , Kirjavainen , V , Pätäri-Sampo , A & Lempiäinen , A 2021 , ' Verification of UriSed 3 PRO automated urine microscope in regional laboratory environment ' , Clinica Chimica Acta , vol. 515 , pp. 96-103 .

Title: Verification of UriSed 3 PRO automated urine microscope in regional laboratory environment
Author: Kouri, Timo; Alagrund, Katariina; Lehtonen, Maaret; Tohmola, Niina; Pihlajamaa, Tero; Kouri, Vesa-Petteri; Friman, Sirpa; Holma, Tanja; Kirjavainen, Vesa; Pätäri-Sampo, Anu; Lempiäinen, Anna
Contributor organization: Medicum
Department of Clinical Chemistry and Hematology
Helsinki University Hospital Area
Department of Diagnostics and Therapeutics
HUS Internal Medicine and Rehabilitation
Date: 2021-04
Language: eng
Number of pages: 8
Belongs to series: Clinica Chimica Acta
ISSN: 0009-8981
Abstract: Background and aims: Ten UriSed 3 PRO automated microscopes (77 Elektronika, Hungary) were verified for nine HUSLAB laboratories with 160 000 annual urine samples. Materials and methods: Particle counting of the primary UriSed 3 PRO instrument (77 Elektronika, Hungary) was verified against reference visual microscopy with 463 urine specimens, and against urine culture on chromogenic agar plates with parallel 396 specimens. Nine secondary instruments were compared pairwise with the primary instrument. Results: Relative imprecisions compared to Poisson distribution, R(CV), were estimated to be 1.0 for white blood cell (WBC) and 1.5 for red blood cell (RBC) counts, respectively. Spearman's correlations against visual microscopy were rS = 0.94 for WBC, rS = 0.87 for RBC, and rS = 0.82 for squamous epithelial cell (SEC) counts. Agreement with visual microscopy (Cohen's weighted kappa) was 0.94 for WBC, 0.89 for RBC, 0.88 for SEC, 0.59 for combined casts, and 0.49 for non-squamous epithelial cells (NEC). Bacteria were detected with a sensitivity of 90% and specificity of 39 against culture at 107 CFB/L (104 CFU/mL). Created flagging limits allowed automated reporting for 70-75% of patient results. Conclusions: UriSed 3 PRO instruments were adopted into routine use after acceptance of the verification.
Subject: Automation
Particle counting
Poisson distribution
Urine sediment
UriSed 3 PRO
318 Medical biotechnology
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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