Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes

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dc.contributor.author Rissanen, Antti J.
dc.contributor.author Saarela, Taija
dc.contributor.author Jäntti, Helena
dc.contributor.author Buck, Moritz
dc.contributor.author Peura, Sari
dc.contributor.author Aalto, Sanni L
dc.contributor.author Ojala, Anne
dc.contributor.author Pumpanen, Jukka
dc.contributor.author Tiirola, Marja
dc.contributor.author Elvert, Marcus
dc.contributor.author Nykänen, Hannu
dc.date.accessioned 2021-05-03T08:23:01Z
dc.date.available 2021-05-03T08:23:01Z
dc.date.issued 2021-02
dc.identifier.citation Rissanen , A J , Saarela , T , Jäntti , H , Buck , M , Peura , S , Aalto , S L , Ojala , A , Pumpanen , J , Tiirola , M , Elvert , M & Nykänen , H 2021 , ' Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes ' , FEMS Microbiology Ecology , vol. 97 , no. 2 , 252 . https://doi.org/10.1093/femsec/fiaa252
dc.identifier.other PURE: 157502976
dc.identifier.other PURE UUID: 3c7be091-f99f-4800-b2dd-a0b65d3824c7
dc.identifier.other WOS: 000637052400009
dc.identifier.other Scopus: 85101027985
dc.identifier.uri http://hdl.handle.net/10138/329586
dc.description.abstract The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajarvi and Lake Lovojarvi. Furthermore, metagenomic analyses were performed to assess the genomic characteristics of methanotrophs in Lovojarvi and the previously studied Lake Alinen Mustajarvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived, especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in clusters of orthologous groups (COG) categories L (replication, recombination and repair) and S (function unknown) were relatively high in metagenome-assembled genomes representing Methylococcales clearly thriving below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. By contrast, genes in COG category N (cell motility) were relatively high in metagenome-assembled genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof FEMS Microbiology Ecology
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 1172 Environmental sciences
dc.subject methanotroph
dc.subject Methylococcales
dc.subject vertical structuring
dc.subject 16S rRNA
dc.subject metagenomics
dc.subject genetic potential
dc.title Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes en
dc.type Article
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Anne Ojala / Principal Investigator
dc.contributor.organization Ecosystem processes (INAR Forest Sciences)
dc.contributor.organization Ecosystems and Environment Research Programme
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1093/femsec/fiaa252
dc.relation.issn 0168-6496
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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