Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance

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Kale , L , Nakurte , I , Jalakas , P , Kunga-Jegere , L , Brosche , M & Rostoks , N 2019 , ' Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance ' , Plant Physiology and Biochemistry , vol. 140 , pp. 18-26 . https://doi.org/10.1016/j.plaphy.2019.05.004

Title: Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance
Author: Kale, Liga; Nakurte, Ilva; Jalakas, Pirko; Kunga-Jegere, Laura; Brosche, Mikael; Rostoks, Nils
Contributor: University of Helsinki, University of Latvia
University of Helsinki, Organismal and Evolutionary Biology Research Programme
Date: 2019-07
Language: eng
Number of pages: 9
Belongs to series: Plant Physiology and Biochemistry
ISSN: 0981-9428
URI: http://hdl.handle.net/10138/329707
Abstract: Arabidopsis thaliana cyclic nucleotide-gated ion channel gene 4 (AtCNGC4) loss-of-function mutant dnd2 exhibits elevated accumulation of salicylic acid (SA), dwarfed morphology, reduced hypersensitive response (HR), altered disease resistance and spontaneous lesions on plant leaves. An orthologous barley mutant, nec1, has been reported to over-accumulate indole-3-acetic acid (IAA) and to exhibit changes in stomatal regulation in response to exogenous auxin. Here we show that the Arabidopsis dnd2 over-accumulates both IAA and abscisic acid (ABA) and displays related phenotypic and physiological changes, such as, reduced stomatal size, higher stomatal density and stomatal index. dnd2 showed increased salt tolerance in root growth assay and significantly reduced stomatal conductance, while maintaining near wt reaction in stomatal conductance upon external application of ABA, and probably consequently increased drought stress tolerance. Introduction of both sid2-1 and fmo1 into dnd2 background resulting in removal of SA did not alter stomatal conductance. Hence, the closed stomata of dnd2 is probably a result of increased ABA levels and not increased SA levels. The triple dnd2sid2abi1-1 mutant exhibited intermediate stomatal conductance compared to dnd2 and abil-1 (ABA insensitive, open stomata), while the response to external ABA was as in abi1-1 suggesting that reduced stomatal conductance in dnd2 is not due to impaired ABA signaling. In conclusion, Arabidopsis dnd2 mutant exhibited ABA overaccumulation and stomatal phenotypes, which may contribute to the observed improvement in drought stress resistance. Thus, Arabidopsis dnd2 mutant may serve as a model for studying crosstalk between biotic and abiotic stress and hormonal response in plants.
Subject: Arabidopsis dnd2
Barley nec1
Auxin
Abscisic acid
Drought stress
LESION MIMIC MUTANTS
GATED ION CHANNELS
BOX PROTEIN TIR1
GENE-EXPRESSION
SALT-STRESS
SIGNALING PATHWAYS
DISEASE RESISTANCE
INNATE IMMUNITY
AIR HUMIDITY
PLANT
1183 Plant biology, microbiology, virology
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