Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance

Show simple item record Kale, Liga Nakurte, Ilva Jalakas, Pirko Kunga-Jegere, Laura Brosche, Mikael Rostoks, Nils 2021-05-08T21:48:02Z 2021-12-18T03:45:26Z 2019-07
dc.identifier.citation Kale , L , Nakurte , I , Jalakas , P , Kunga-Jegere , L , Brosche , M & Rostoks , N 2019 , ' Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance ' , Plant Physiology and Biochemistry , vol. 140 , pp. 18-26 .
dc.identifier.other PURE: 126097306
dc.identifier.other PURE UUID: bdcd08a2-02a9-4031-ae5a-82b8a2239719
dc.identifier.other WOS: 000471736300003
dc.identifier.other ORCID: /0000-0002-1135-2496/work/60611617
dc.description.abstract Arabidopsis thaliana cyclic nucleotide-gated ion channel gene 4 (AtCNGC4) loss-of-function mutant dnd2 exhibits elevated accumulation of salicylic acid (SA), dwarfed morphology, reduced hypersensitive response (HR), altered disease resistance and spontaneous lesions on plant leaves. An orthologous barley mutant, nec1, has been reported to over-accumulate indole-3-acetic acid (IAA) and to exhibit changes in stomatal regulation in response to exogenous auxin. Here we show that the Arabidopsis dnd2 over-accumulates both IAA and abscisic acid (ABA) and displays related phenotypic and physiological changes, such as, reduced stomatal size, higher stomatal density and stomatal index. dnd2 showed increased salt tolerance in root growth assay and significantly reduced stomatal conductance, while maintaining near wt reaction in stomatal conductance upon external application of ABA, and probably consequently increased drought stress tolerance. Introduction of both sid2-1 and fmo1 into dnd2 background resulting in removal of SA did not alter stomatal conductance. Hence, the closed stomata of dnd2 is probably a result of increased ABA levels and not increased SA levels. The triple dnd2sid2abi1-1 mutant exhibited intermediate stomatal conductance compared to dnd2 and abil-1 (ABA insensitive, open stomata), while the response to external ABA was as in abi1-1 suggesting that reduced stomatal conductance in dnd2 is not due to impaired ABA signaling. In conclusion, Arabidopsis dnd2 mutant exhibited ABA overaccumulation and stomatal phenotypes, which may contribute to the observed improvement in drought stress resistance. Thus, Arabidopsis dnd2 mutant may serve as a model for studying crosstalk between biotic and abiotic stress and hormonal response in plants. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Plant Physiology and Biochemistry
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Arabidopsis dnd2
dc.subject Barley nec1
dc.subject Auxin
dc.subject Abscisic acid
dc.subject Drought stress
dc.subject BOX PROTEIN TIR1
dc.subject SALT-STRESS
dc.subject AIR HUMIDITY
dc.subject PLANT
dc.subject 1183 Plant biology, microbiology, virology
dc.title Arabidopsis mutant dnd2 exhibits increased auxin and abscisic acid content and reduced stomatal conductance en
dc.type Article
dc.contributor.organization Organismal and Evolutionary Biology Research Programme
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Plant stress and natural variation
dc.contributor.organization Plant Biology
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0981-9428
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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