Protocol: Adeno-Associated Virus-Mediated Gene Transfer in Ex Vivo Cultured Embryonic Mammary Gland

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dc.contributor.author Lan, Qiang
dc.contributor.author Mikkola, Marja L.
dc.date.accessioned 2021-05-12T11:17:01Z
dc.date.available 2021-05-12T11:17:01Z
dc.date.issued 2020-12
dc.identifier.citation Lan , Q & Mikkola , M L 2020 , ' Protocol: Adeno-Associated Virus-Mediated Gene Transfer in Ex Vivo Cultured Embryonic Mammary Gland ' , Journal of Mammary Gland Biology and Neoplasia , vol. 25 , no. 4 , pp. 409-416 . https://doi.org/10.1007/s10911-020-09461-4
dc.identifier.other PURE: 150048722
dc.identifier.other PURE UUID: cd287103-3d95-4c75-a26c-7c6cf9e869ba
dc.identifier.other RIS: urn:7EBF8A983C3C8E9156569C9CAB6F2CD0
dc.identifier.other RIS: Lan2020
dc.identifier.other WOS: 000574725100001
dc.identifier.uri http://hdl.handle.net/10138/329896
dc.description.abstract Branching morphogenesis of the murine mammary gland starts during late embryogenesis. It is regulated by the signals emanating both from the epithelium and the mesenchyme, yet the molecular mechanisms regulating this process remain poorly understood. We have previously developed a unique whole organ culture technique for embryonic mammary glands, which provides a powerful tool to monitor and manipulate branching morphogenesis ex vivo. Nowadays, RNA sequencing and other transcriptional profiling techniques provide robust methods to identify components of gene regulatory networks driving branching morphogenesis. However, validation of the candidate genes still mainly depends on the use of the transgenic mouse models, especially in mammary gland studies. By comparing different serotypes of recombinant adeno-associated virus (rAAVs), we found out that rAAVs provide sufficient efficiency for gene transfer with different tissue preferences depending on the serotypes of the virus. AAV-2 and AAV-8 preferentially target epithelial and mesenchymal compartments, respectively, while AAV-9 infects both tissues. Here, we describe a protocol for AAV-mediated gene transfer in ex vivo cultured murine embryonic mammary gland facilitating gene function studies on mammary gland branching morphogenesis. en
dc.format.extent 8
dc.language.iso eng
dc.relation.ispartof Journal of Mammary Gland Biology and Neoplasia
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject Organ culture
dc.subject Ex vivo
dc.subject Explant culture
dc.subject Adeno-associated virus
dc.subject MOUSE
dc.subject MORPHOGENESIS
dc.subject APPENDAGE
dc.title Protocol: Adeno-Associated Virus-Mediated Gene Transfer in Ex Vivo Cultured Embryonic Mammary Gland en
dc.type Review Article
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Helsinki Institute of Life Science HiLIFE, Joint Activities
dc.contributor.organization Biosciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1007/s10911-020-09461-4
dc.relation.issn 1083-3021
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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