Repatterning of the inflorescence meristem in Gerbera hybrida after wounding

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Zhang , T , Wang , F & Elomaa , P 2021 , ' Repatterning of the inflorescence meristem in Gerbera hybrida after wounding ' , Journal of Plant Research , vol. 134 , pp. 431-440 . https://doi.org/10.1007/s10265-021-01253-z

Title: Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
Author: Zhang, Teng; Wang, Feng; Elomaa, Paula
Contributor: University of Helsinki, Plant Production Sciences
University of Helsinki, Asteraceae developmental biology and secondary metabolism
University of Helsinki, Department of Agricultural Sciences
Date: 2021-05
Language: eng
Number of pages: 10
Belongs to series: Journal of Plant Research
ISSN: 0918-9440
URI: http://hdl.handle.net/10138/330276
Abstract: The Asteraceae plant family is characterized by inflorescences, called flower heads or capitula that may combine hundreds of individual florets into a single flower-like structure. The florets are arranged in a regular phyllotactic pattern with Fibonacci numbers of left- and right-winding spirals. Such a pattern may be disrupted due to physical constraints or by wounding occur-ring during the early meristem development. Recovery from wounding re-establishes patterning although the mechanisms have remained elusive. In this study, we applied Gerbera hybrida as a model system and established methods to conduct wounding experiments either with syringe needles or using laser ablation combined with live imaging of head meristems. By revisiting the historical experiments in sunflower, we conducted wounding to transgenic auxin reporter lines of gerbera and followed the recovery of cellular growth and meristem patterning. We show that wounding disrupted the expression of the gerbera CLAVATA3 (GhCLV3) gene that marks the undifferentiated meristematic region and led to de novo re-initiation of patterning at the wound margin. During the recovery growth, three to five layers of elongated cells showing periclinal cell division planes and lacking auxin signal were formed at the wound rim. DR5 auxin signal was shown to localize and form regularly spaced maxima in a distance from the wound rim. Consequently, spiral pattern of contact parastichies was re-established by stacking of new auxin maxima on top of the previous ones. The developed methods facilitate future studies on understanding the molecular mechanisms of de novo patterning of meristems.
Subject: Asteraceae
Auxin
Flower head
Laser ablation
Phyllotaxis
Wounding
11831 Plant biology
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